卵巢透明细胞癌中pVHL的诊断价值和意义

Diagnostic value and significance of pVHL in ovarian clear cell carcinoma

目的观察p VHL在卵巢不同类型上皮性癌中的表达,探讨p VHL在卵巢透明细胞癌诊断价值。方法采用免疫组化En Vision法检测卵巢恶性上皮性癌p VHL的表达。结果 20例卵巢透明细胞癌均弥漫强(+),阳性率为90%;20例卵巢高级别浆液性癌中3例p VHL呈胞质/胞膜(+),阳性率15%;14例卵巢高-中分化宫内膜样腺癌有2例(+),阳性率14.3%,p VHL在浆液性癌和子宫内膜样腺癌中的阳性率明显低于透明细胞癌(p<0.01)。p VHL对卵巢透明细胞癌的敏感性90%,特异性85.2%。结论 p VHL是一种敏感性高的卵巢透明细胞癌标记物,p VHL弥漫强(+)有助于辅助诊断卵巢透明细胞癌,具有潜在的临床价值。

Objective To study the expression of von Hippel-Lindau（ p VHL） in different types of ovarian carcinomas and to explore the diagnostic value of p VHL in ovarian clear cell carcinomas. Methods Immunohistochemical En Vision method was used to detect the expression in ovarian malignant epithelial carcinom. Results 20 clear cell carcinomas were almost diffusely strong expression of p VHL,and the positive rate was 90%. In 20 cases of ovarian high grade serous carcinoma,p VHL was cytoplasmic/membranous positive expression in 3 cases,the positive rate was 15%. 2 of 14 cases of ovarian moderately/well differentiated endometrioid adenocarcinoma were positively expressed,and the positive rate was14. 3%. The expression of p VHL in serous carcinoma and endometrioid adenocarcinoma was significantly lower than that in ovarian clear cell carcinoma; the difference was statistical significance（ P〈 0. 01）. The sensitivity and specificity of p VHL in the diagnosis of ovarian clear cell carcinoma were about 90% and 85. 2%,respectively. Conclusion p VHL may be a highly sensitive marker of ovarian clear cell carcinoma. The overexpression of p VHL may be helpful in the diagnosis of ovarian clear cell carcinomas and it has potential clinical value.