右归饮对去势雌性小鼠骨质疏松的治疗作用

Therapeutic effect of Yougui Drink on osteoporosis in ovariectomized female mice

目的探讨右归饮对去势雌性小鼠骨质疏松的影响,并对其作用机制进行研究。方法将C57BL/6雌性小鼠去卵巢手术模拟骨质疏松症,饲养12周后,分别分为假手术组、模型组、雷洛昔芬组、右归饮组,每组各9只,模型组、假手术组ig生理盐水,右归饮组ig右归饮溶液。雷洛昔芬组ig盐酸雷洛昔芬溶液10 mg/kg。每天每只均给药0.2 m L,连续给药5周,眼球取血,测定血清中生化指标和钙、磷水平;将动物处死,测定骨形态计量指标和做Micro-CT。分离并培养骨内间充质干细胞,测定细胞的增殖和分化。结果与模型组比较,右归饮组的骨密度、骨体积分数、骨小梁平均厚度、骨小梁数量均显著升高,骨表面积/骨体积和骨小梁分离度均显著降低,差异具有统计学意义(P<0.05)。右归饮组I型胶原氨基端前肽(P1NP)水平较模型组显著升高(P<0.05),胶原羧基端(CTx)水平比模型组略高一点,差异均无统计学意义。右归饮组钙、磷水平与模型组各指标之间的差异均无统计学意义。与模型组比较,右归饮组细胞呈增长趋势,但增长率较其他组相对较低(P<0.01),倍数变化显著升高(P<0.01)。结论右归饮对去势雌性小鼠骨质疏松有治疗作用,可能是通过促进间充质干细胞分化成成骨细胞,从而刺激骨形成,达到有效抑制小鼠骨的丢失。

Objective To explore the therapeutic effect of Yougui Drink on osteoporosis in ovariectomized female mice and study its mechanisms. Methods The osteoporosis was mimicked by excising the ovary of C57BL/6 mice. After 12 weeks, random allocation was taken to averagely divide into Sham, model, raloxifene, and Yougui Drink e groups, and each group had 9 mice. Rats in the control group and model group were given the same volume of normal saline. Rats in Yougui Drink group were given Yougui Drink. Rats in raloxifene group were ig administered with Raloxifene Hydrochloride 10 mg/kg. All animals were given the volume 0.2 mL, 1 time/d, and treated for 5 weeks. The blood samples were taken from the eyeball, and serum biochemical indexes and levels of calcium and phosphorus were assessed. Mice were killed, and bone histomorphometry were assessed by Micro-CT. Bone mesenchymal stem cells were isolated and cultured and cell proliferation and ability of osteogenic differentiation were assessed. Results Compared with model group, systemic manifestations score, bone mineral density, bone volume fraction, the mean trabecular thickness, trabecular number in the Yougui Drink group were significantly increased, but bone surface area / bone volume and trabecular separation were significantly decreased （P 〈 0.05）. The levels of P1NP in the Yougui Drink group were significantly increased （P 〈 0.05）, but the CTx levels were slightly higher than those in the model group, the differences were not statistically significant. There were no significant differences on levels of calcium and phosphorus between the Yougui Drink group and the model group. Compared with the model group, the cells in the Yougui Drink group were increased, but the growth rates were lower than those in the other groups （P 〈 0.01）, and the changes of multiples were significantly higher （P 〈 0.01）. Conclusion Yougui Drink can treat osteoporosis in ovariectomized female mice, which may promote the differentiation of mesenchymal stem cells into osteoblasts, thereby stimulate bone formation, and effectively inhibit bone loss in mice.