非小细胞肺癌中NUSAP1的表达及临床意义

Expression and clinical significance of NUSAP1 in non-small cell lung cancer

目的探讨非小细胞肺癌（NSCLC）组织中核仁纺锤体相关蛋白1（NUSAP1）的表达情况，分析其与患者临床病理特征的关系。方法收集新鲜NSCLC肿瘤组织及癌旁组织各10例，采用实时定量PCR和Western blot分别检测NUSAP1mRNA和蛋白的表达；另收集69例NSCLC患者手术切除的癌组织及癌旁组织，采用免疫组织化学染色检测NUSAP1的表达情况，并分析其表达与患者临床病理特征的关系。结果NSCLC组织中NUsAP1mRNA及蛋白表达均显著高于癌旁组织（t值分别为3．401、31．89，P值均〈0．05）；NUSAP1在NSCLC组织中的高表达率为59．42％，而在癌旁组织中的高表达率为15．94％，差异有统计学意义（χ2=27．77，P〈0．05）；NUSAP1在NSCLC组织中高表达与患者性别、年龄、TNM分期无关，但与肿瘤大小及有无淋巴结转移有关（χ2值分别为5．445、18．034，P值均〈0．05）。结论NUSAPI在NSCLC组织中呈异常高表达，可能与NSCLC的发生、发展相关。

Objective To investigate the expression and clinical significance of nucleolar and spindle-associated protein 1 （NUSAP1） in non-small cell lung cancer （NSCLC）. Methods Ten cases of fresh NSCLC tissues and adjacent tissues were collected to detect the expressions of NUSAP1 mRNA and protein by quantitative real-time polymerase chain reaction and Western blot. 69 cases of NSCLC tissues and adjacent tissues were collected to detect the expression of NUSAP1 by immunohistochemical staining, and analyze the relationship between the expression of NUSAP1 and clinicopathological features of NSCLC. Results The expressions of NUSAP1 mRNA and protein in NSCLC tissues were significantly higher than those in adjacent tissues （ t = 3. 401, 31.89, all P % 0.05）. The high expression rate of NUSAP1 was 59.42% in NSCLC tissues, and that was 15.94% in adjacent tissues, the difference was statistically significant （ χ2 =27.77, P〈0.05）. NUSAP1 overexpression was not associated with gender, age and TNM stage, but was correlated with tumor size and lymphatic metastasis of NSCLC （ χ2=5. 445, 18. 034, all P 〈0.05）. Conclusions NUSAP1 is overexpressed in NSCLC tissues and may be related to the occurrence and development of NSCLC.