青海省果洛藏族自治州藏系绵羊血清鼠疫F1抗体流行病学调查

Epidemiological investigation of plague F1 antibody in Tibetan sheep serum in Guoluo Prefecture, Qinghai Province

目的对青海省果洛藏族自治州藏系绵羊进行血清鼠疫F1抗体检测，了解当地藏系绵羊鼠疫流行情况。方法2014、2015年在果洛藏族自治州的玛沁县、玛多县、甘德县、班玛县、久治县、达日县。采集藏系绵羊颈静脉全血分离血清，应用鼠疫间接血凝试验（IHA）、胶体金纸上色谱（GICA）法进行鼠疫F1抗体血清学检测。结果共采集藏系绵羊血清1481份，其中玛沁县566份、玛多县315份、甘德县150份、班玛县150份、久治县150份、达日县150份。检出鼠疫F1抗体阳性血清14份，阳性率为0.95％（14／1481），全部来源于玛沁县。结论果洛藏族自治州存在藏系绵羊鼠疫的流行，应加强藏系绵羊鼠疫的防治监测工作。

Objective To understand the epidemic trend of Tibetan sheep plague in Guoluo Prefecture, Qinghai Province, we detected the plague F1 antibody in Tibetan sheep serum in this area. Methods Indirect hemagglutination test （IHA） and colloidal gold immunochromatography （GICA） were applied to test serum samples of Tibetan sheep which were separated from 5 ml whole blood drew from jugular vein in Maqin County, Maduo County, Gande County, Banma County, Jiuzhi County and Dari County in 2014 and 2015. Results We collected 1 481 serum samples, 566 from Maqin County, 315 from Maduo County, 150 from Gande County, 150 from Banma County, 150 from Jiuzhi County and 150 from Dari County. Totally 14 serum samples showed F1 antibody positive, the positive rate was 0.95% （14/1 481）, and they were all from Maqin County. Conclusions This area has the prevalence of Tibetan sheep plague. Therefore, the monitoring work of Tibetan sheep plague should be strengthened.