冬凌草甲素联合卡培他滨对人乳腺癌MDA-MB-231细胞增殖的影响

Effects of Oridonin combined with Capecitabine on the proliferaction of MDA-MB-231 human breast cancer cens

目的观察冬凌草甲素联合卡培他滨对乳腺癌MDA-MB-231细胞增殖的影响。方法观察不同浓度（10、20及40μmol／L）的冬凌草甲素和卡培他滨及其两者联合用药与MDA-MB-231细胞孵育24和48h的细胞存活率。考察5μmol／L的冬凌草甲素和卡培他滨及其两者联合使用对MDA。MB-231细胞集落形成的影响。考察20μmol／L的冬凌草甲素和卡培他滨及其两者联合使用对MDA-MB-231细胞核形态，细胞周期及凋亡的影响。结果冬凌草甲素和卡培他滨在20μmol／L孵育48h对MDA-MB-231细胞的抑制率分别为49．5％和58．6％，而将两者联合使用时，对肿瘤的抑制率达到94．6％。联合给药后肿瘤集落形成的大小和个数较单独给药时明显减少（P〈0．01）。同时给予冬凌草甲素和卡培他滨，可将细胞同时阻滞于S期和G2／M期，凋亡细胞的比例也有显著升高（P〈0．01）。结论将冬凌草甲素与卡培他滨联合给药之后，两者可以协同起效，共同发挥抗肿瘤效果，对乳腺癌MDA-MB-231细胞的增殖起到显著的抑制作用。

Objective To investigate the effects of oridonin combined with capecitabine on the proliferaction of MDA-MB-231 human breast cancer ceils. Methods Effect of different concentrations （ 10, 20 and 40 μmol/L）of oridonin, capecitahine and their combination on the proliferation of MDA-MB-231 celts after incubation for 24 or 48 h was studied. Then, the effect of 5 μmol/L of oridonin, capecitabine and their combination on cell colony formation was detected. Finally, influence of 20 μmol/L of oridonin, capecitabine and their combination on morphological alteration of nucleus, cell cycle and apoptosis was explored. Results The inhibition rate on MDA-MB-231 cells after incubation with 20 μmoL/L oridonin or capecitabine for 48 h was 49. 5% and 58.6%, respectively, while the inhibition rate against proliferation of MDA-MB-231 cells reached 94. 6% with combination of 20 txmol/L oridonin and capecitabine. Cells incubated with combination of oridonin and capecitabine formed fewer and smaller colonies （ P 〈 0. 01 ）. Meanwhile, cells in the combination group arrested at S and G2/M phases at the same time, and combination of two drugs caused more apoptotic ceils （ P 〈 0. 01 ）. Conclusion Oridonin combined with capeeitabine can synergistically inhibit the proliferation of MDA-MB-231 human breast cancer cells.