响应面法优化微波辅助提取黑加仑多酚工艺及其抗氧化活性研究

Optimization of microwave-assisted extraction of polyphenols from blackcurrant by response surface method and analysis of antioxidant activity of the extract

采用微波辅助提取黑加仑多酚,在微波温度、乙醇浓度、微波时间和微波功率四个单因素试验的基础上,利用响应面法对黑加仑多酚的提取工艺条件进行优化,并对黑加仑多酚提取液的抗氧化活性进行了研究。结果表明,微波辅助提取黑加仑多酚的最佳工艺条件是:微波温度50℃,乙醇浓度40%,微波时间4 min,微波功率700 W。在此条件下,黑加仑多酚得率为1.58%。黑加仑多酚提取液对ABTS自由基、DPPH自由基、羟基自由基的平均清除率分别为98%、86%,93%,黑加仑多酚提取液对ABTS自由基、DPPH自由基和羟基自由基具有良好的清除能力。

Microwave-assisted method was used to extract polyphenols from blackcurrant.The extraction process was optimized by response surface method on the basis of four single factor tests which are microwave temperature,ethanol concentration,microwave time and microwave power.In addition the antioxidant activity of polyphenols that were extracted from blackcurrant was studied.The optimal extraction conditions were as follows ：microwave temperature at 50℃,ethanol concentration with 40%,microwave duration of 4 min,and microwave power of 700 W.Under the optimal conditions,the yield of polyphenols was 1.58%.The average inhibition rate of polyphenols was evaluated by testing ABTS free radical,DPPH free radical and hydroxyl free radical,which were 98%,86% and 93%,respectively.In conclusion,the polyphenols extracted from blackcurrant had strong scavenging ability for ABTS free radical,DPPH free radical and hydroxyl free radical.