白藜芦醇诱导并增加顺铂诱导肝癌细胞凋亡的机制研究

Mechanism study of resveratrol-induced and increasing cisplatin-induced apoptosis in hepatocellular carcinoma cells

目的探讨白藜芦醇抑制肝癌细胞增殖并对顺铂诱导肝癌细胞凋亡的增敏机制。方法将肝癌Hep G2细胞培养传代后根据给药方式分为对照组、实验组1(10μg/m L Res)、实验组2(5μg/m L Pt)、联合用药组(10μg/m L Res+5μg/m L Pt),比较四组肝癌细胞增殖与凋亡情况、Caspase 3活性以及Bcl-2、Bax蛋白表达。结果实验组1、实验组2、联合用药组药物作用于肝癌细胞系24 h后,相对生长率与对照组相比,差异显著,且联合用药组低于其他三组(P<0.01)。联合用药组细胞凋亡率高于实验组1、实验组2(P<0.05)。实验组1、实验组2 Caspase 3活性高于对照组,且联合用药组高于其他三组(P<0.05)。ELISA法和Western-blot显示,联合用药组细胞内Bcl-2、Bax蛋白含量与其他三组比较,差异有统计学意义(P<0.05)。结论白藜芦醇诱导肝癌细胞凋亡,并促进顺铂诱导肝癌细胞凋亡,从而抑制肝癌细胞增殖。

Objective To investigate the mechanism of resveratrol in inhibiting the proliferation of hepatocellular carcino-ma cells and increasing the apoptosis induced by cisplatin. Methods The Hep G2 cells were divided into control group, experimental group 1（10 μg/m L Res）, experimental group 2（5 μg/m L Pt） and combination group（10 μg/m L Res＋5 μg/m L Pt） according to disposal methods. The proliferation and apoptosis rates of hepatocellular carcinoma cells, Caspase 3 activity, Bcl-2 and Bax protein expression were compared between the four groups. Results After drugs used 24 h in hepatoma cells, compared with the control group, the relative growth rates of hepatoma cells in experimental group 1, experimental group 2 and combina-tion group showed significant differences, and that of the combination group was lower than the other three groups（ P〈0.01）.The apoptosis rate in the combination group was higher than that in the experimental group 1 and experimental group 2（ P〈0.05）. The Caspase 3 activity of experimental group 1 and experimental group 2 were significantly higher than that of the control group, and that of the combination group was higher than the other three groups（ P〈0.05）. The results of ELISA and Western-blot showed that the content of Bcl-2 and Bax in the combination group were significantly different from those in the other three groups（P〈0.05）. Conclusion Resveratrol induces apoptosis of hepatoma cells and promotes cisplatin-induced apoptosis in hepatocellular carcinoma, thus inhibits the proliferation of hepatocellular carcinoma cells.