瑞香素对2型糖尿病大鼠血清炎症因子及血糖水平的影响

Effect of daphnetin on levels of serum inflammatory cytokines and blood glucose in type 2 diabetic rats

目的观察瑞香素对2型糖尿病（T2DM）大鼠血清炎症因子水平的影响以及对血糖、血清胰岛素的作用，探讨瑞香素对改善糖尿病大鼠胰岛素抵抗的作用机理。方法予健康雄性Wistar大鼠高糖高脂饲料喂养30天后，腹腔注射链脲佐菌素（STZ）40mg/kg制备T2DM大鼠模型，将造模成功大鼠随机分为模型组（12例）和瑞香素组（12例），同时将正常大鼠随机分为正常组（12例）和对照组（12例）。分别予对照组和瑞香素组大鼠腹腔注射瑞香素每天4mg／kg，共注射30天，检测各组大鼠血清炎症因子、空腹血糖（FPG）和空腹胰岛素（FINS）水平，并计算胰岛素抵抗指数（HOMA-IR）。结果与正常组相比，模型组大鼠血清白细胞介素（IL）-6和肿瘤坏死因子（TNF）-α均明显升高（P〈0．01），FPG、FINS水平以及HOMA—IR也明显升高（P〈0．01）。瑞香素组大鼠的各项指标较模型组均明显降低（P〈0．05）。正常组与对照组上述指标比较差异无统计学意义（P〉0．05）。结论瑞香素可能通过降低T2DM大鼠血清炎症因子水平从而起到降低血糖和改善胰岛素抵抗的作用。

Objective To observe the effects of daphnetin on the serum levels of serum imflam- matory cytokines ,blood glucose and insulin in rats with type 2 diabetes mellitus （T2DM） and to explore the mechanism for its improvement on insulin resistance of rats. Methods T2DM rat model was induced by intravenous injection of streptozotocin（40 mg/kg） after 30 days with high glucose and high fat diet. T2DM model rats were randomized into the model group （n = 12 ） and the daphnetin group （n = 12 ）. Meanwhile,normal rats were assigned to the normal group（ n = 12） and the control group（ n = 12）. The daphnetin group and the control group were injected intraperitoneally with daphnetiu 4 mg/kg once a day for 30 days. And then serum imflammatory cytokines, fasting plasma glucose （FPG）, fasting insulin （FINS） and insulin resistance index （ HOMA-IR ） of all the rats were measured. Results Compared with the normal group, interleukin-6 （IL-6） and tumor necrosis factor-α （TNF-α） of the model group were significantly bigher（P 〈 0.01 ） as well as FPG, FINS and HOMA-IR （P 〈 0.01 ）. Compared with the model group, above indexes were significantly lower in the daphnetin group （ P 〈 0.05 ）. No significant differences of above indexes between the normal group and the control group were found （ P 〉 0.05 ）. Conclusion Daphnetin can lower blood glucose and reduce insulin resistance by decreasing the level of inflammatory cytokines in T2DM rats.