补肾生髓方对D-半乳糖衰老模型大鼠学习记忆能力及衰老相关蛋白P16、P21的影响

Effect of Bushen Shengsui prescription on learning and memory abilities and senescence-associated proteins P16 and P21 in a rat model of senescence induced by D-galactose

目的:探讨补肾生髓方对衰老大鼠学习记忆能力及衰老相关蛋白P16、P21的影响。方法:将50只实验大鼠随机分成D-半乳糖衰老模型组、正常组、补肾生髓方组、六味地黄丸组、维生素E组共5个组。采用颈背部皮下注射D-半乳糖400mg/kg,连续4个月,制备衰老模型,同时给予相应药物灌胃,正常组给予等剂量0.9%氯化钠注射液。采用Morris水迷宫检测大鼠学习记忆能力,Western blot检测大鼠海马中P16、P21蛋白的表达。结果:补肾生髓方能够缩短D-半乳糖衰老大鼠到达平台的时间,降低衰老模型大鼠海马中P16、P21的含量。结论:补肾生髓方能够提高衰老大鼠的学习记忆能力,通过降低大鼠体内P16、P21的含量从而达到延缓衰老的目的。

Objective：To investigate the effect of Bushen Shengsui prescription on learning and memory abilities and senescence - associated proteins P16 and P21 in a rat model of D - galactose - induced senescence. Methods：A total of 50 experimental rats were randomly divided into D - galactose - induced senescence model group, normal group, Bushen Shengsui prescription group, Liuwei Dihuang pill group, and vitamin E group. Subcutaneous injection of D - galactose at the back of the neck was performed for four consecutive months to establish a rat model of senescence and the corresponding drug was given by garage ;the rats in the normal group were given 0.9% sodium chloride injection at the same dose. The Morris water maze test was used to evaluate learning and memory abilities, and Western blot was used to measure the pro- tein expression of P16 and P21 in the hippocampus. Results：Bushen Shengsui prescription significantly shortened the time to reach the platform and reduced the content of P16 and P21 in the hippocampus in rats with D - galactose - induced se- nescence. Conclusion：Bushen Shengsui prescription can improve the learning and memory abilities of senescent rats and delay senescence by reducing the content of P16 and P21.