长春西汀减轻脑出血大鼠脑组织的炎症损伤

Effects of vinpocetine on inflammation of brain in rats with intracerebral hemorrhage

目的:探讨长春西汀对脑出血大鼠的干预作用以及对炎症损伤的影响。方法:将大鼠随机分为假手术组、脑出血组以及长春西汀低剂量组、中剂量组和高剂量组。除假手术组外,其余大鼠均通过注射VII型胶原酶的方法建立脑出血模型,长春西汀低剂量组、中剂量组和高剂量组分别给予腹腔注射0.5、1.0和1.5 mg/kg长春西汀,每天1次,共给药7 d。给药完成后,对各组大鼠进行神经功能缺损评分,称重法计算脑组织的含水量,检测脑组织中髓过氧化物酶(MPO)活性,Western blot法检测脑组织中Toll样受体4(TLR4)、核因子κB(NF-κB)、细胞间黏附分子1(ICAM-1)与血管细胞黏附分子1(VCAM-1)的蛋白表达。结果:与脑出血组相比,给予长春西汀干预的大鼠神经功能缺损评分显著下降(P<0.05),脑组织含水量明显下降(P<0.05),MPO活性显著降低(P<0.05),脑组织中TLR4、NF-κB、ICAM-1与VCAM-1的蛋白表达显著下降(P<0.05)。结论:长春西汀对脑出血大鼠脑组织具有保护作用,可能是通过抑制TLR4诱导的NF-κB信号通路以及ICAM-1与VCAM-1的表达来抑制炎症反应。

AIM： To investigate the effects of vinpocetine on inflammation of brain in intracerebral hemorrhage （ICH） rats and to explore the underlying mechanisms. METHODS： All rats were randomly divided into sham group, ICH group, ICH with low dose of vinpocetine treatment group, ICH with medium dose of vinpocetine treatment group, and ICH with high dose of vinpocetine treatment group. Except sham group, the rats in other groups were injected with type VII col- lagenase to establish ICH model, and then the rats in vinpocetine treatment groups were received vinpocetine at 0. 5 , 1. 0 or1. 5 mg/kg by intraperitoneal injection once a day for 7 days. After corresponding treatment, the impairment of neurological function in the rats was scored and the water content of the brain tissues was measured. Moreover, the activity of myeloper-oxidase （MPO） was determined by ELISA, and the protein expression of Toll-like receptors 4 （TLR4） , nuclear factor-kB （ NF-κB） , intercellular cell adhesion molecule-1 （ ICAM-1 ） and vascular cell adhesion molcule-1 （ VCAM-1 ） in the brain tissues was determined by Western blot. RESULTS： Compared with ICH group, vinpocetine treatment significantly de-creased the scores of the impairment of neurological function and the water content of the brain tissues. Moreover, the activ-ity of MPO and the protein expression of TLR4, NF-kB, ICAM-1 and VCAM-1 were also reduced after vinpocetine treat-ment （P 〈 0. 05） . CONCLUSION： Vinpocetine improves neurological function in ICH rats via suppression of inflamma-tion by inhibiting NF-kB signaling and expression of ICAM-1 and VCAM-1.