急性胰腺炎大鼠胰腺细胞骨架F-actin与再生因子RegⅠ相关性研究

Correlation between cytoskeleton F-actin and regenerative factor RegⅠ in acute pancreatitis ratsL

目的:研究L-精氨酸诱导大鼠急性胰腺炎胰腺细胞骨架F-actin与再生因子RegⅠ的水平变化情况及相关性。方法:将SD大鼠随机分为空白组、对照组、模型组,通过腹腔注射L-精氨酸诱导急性胰腺炎模型后将两组分别选取12h、24h、48h、72h时间点观察胰腺大体病理变化,通过western blot检测各组F-actin与RegⅠ的含量。结果:western-blotting检测模型组F-actin相对表达量与空白组、对照组相比差异有统计学意义(P<0.05),模型组RegⅠ相对表达量较空白组、对照组明显升高,且差异有统计学意义(P<0.05)。结论:腹腔注射L-精氨酸诱导的急性胰腺炎胰腺细胞骨架F-actin与再生因子RegⅠ可能呈负相关。

Objective: To investigate the changes and correlation of cytoskeleton F-actin and regenerative factor RegⅠ in pancreas tissue of rats with acute pancreatitis induced by L-arginine. Methods: SD rats( n = 45)were randomly divided into blank group,control group and model group. Acute pancreatitis was induced by intraperitoneal injection of L-arginine,then the rats were sacrificed at 12 h,24h,48 h and 72 h. The levels of F-actin and RegⅠ were detected by western blot. Results: The relative expression of F-actin in model group was significantly lower than those in blank group and control group( P < 0. 05),and the relative expression of RegⅠ in model group was significantly higher than those in blank group and control group( P < 0. 05). Conclusion: Cytoskeleton F-actin may be negatively correlated with RegⅠ in acute pancreatitis induced by intraperitoneal injection of L-arginine.