摘要为研究利用当地分离菌株制备鸭巴氏杆菌病疫苗的方法,本试验从泰州地区疑似巴氏杆菌病的病死鸭中分离出巴氏杆菌,经鉴定合格后作为菌种。用马丁肉汤培养后获得菌液,甲醛灭活,加油佐剂制成鸭巴氏杆菌油佐剂灭活苗。无菌检验,安全检验合格;效力检验中,免疫接种剂量为1 m L时,免疫保护率为100%,表明本次所制备疫苗安全有效。
9Clinical and laboratory standards institute(CLSI). Performance standards for antimicrobial susceptibility testing twentieth informational supplement[M]. USA, 2010.
10Mbuthial P G, Njagi L W, Nyaga P N, et al. Pasteurella multocida in scavenging family chickens and ducks: carrier status, age susceptibility and transmission between species[J]. Avian Pathology, 2008,37(1) :51-57.