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新城疫病毒在鸡胚成纤维细胞中的培养及纯化

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摘要 利用鸡胚成纤维细胞对新城疫毒株GM0511株进行增殖培养和蚀斑法纯化,通过对最适细胞密度、病毒最佳接种浓度、覆盖琼脂糖浓度、蚀斑形成时间等条件进行优化摸索,最终获得产斑时间一致,蚀斑大小一致、出斑整齐的纯化新城疫毒株,并使原病毒血凝效价提高2个滴度,为后续实验奠定基础。
出处 《浙江农业科学》 2017年第12期2265-2267,共3页 Journal of Zhejiang Agricultural Sciences
基金 山东省农业科学院院青年科研基金(2014QNM15 2015YQN61 2016YQN56) 山东省自然科学基金(ZR2015CM009)
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