摘要
首先研究了添加玉米浆和表面活性剂吐温对谷氨酸棒状杆菌GKGD发酵生产α-酮戊二酸的影响.在此基础上,通过采用添加过量玉米浆,同时在产酸期添加吐温60的培养方法,显著提高了α-酮戊二酸的产量.结果表明:相比于吐温80,吐温60更有利于α-酮戊二酸的分泌;在初始发酵培养基中添加6 mL/L玉米浆,在发酵10 h加入1 mL/L吐温60,可以使α-酮戊二酸的质量浓度达到17.9 g/L.
In this study,the influence of corn steep liquor and surfactants addition on fermentative production of α-ketoglutarate by Corynebacterium glutamicum GKGD was investigated.The production ofα-ketoglutarate was significantly improved by adding corn steep liquor in the initial fermentation medium and adding appropriate amount of Tween 60 at the beginning of acid-producing phase.The results indicated that Tween 60 was more effective in promoting the secretion of α-ketoglutarate than Tween 80.The α-ketoglutarate production achieved the highest value of 17.9 g/L with 6 mL/L of corn steep liquor and 1 mL/L of Tween 60 added.
出处
《发酵科技通讯》
CAS
2017年第4期216-219,共4页
Bulletin of Fermentation Science and Technology
基金
中国博士后科学基金资助项目(2016M601269)
