摘要
运用根据香菇全基因组设计的104对SSR引物,对香菇21株原生质体单核体进行了遗传多样性分析,有58对SSR引物表现出多态性,多态性引物比例为55.8%,获得了430条扩增条带,其中多态性条带占298条,多态性条带比例达69.3%.运用NTSYS-pc21软件分析了供试菌株之间的遗传相似性,研究表明:栽培品种和野生菌株之间存在明显的遗传差异性,但具有很高的相似系数的栽培品种之间则存在很近的亲缘关系;同一双核体得到的两个原生质体单核体菌株之间存在非常近的遗传关系.
By using 104 pairs of SSR primers designed from the whole genome of Lentinula edodes,the genetic diversity of 21 protoplasted monokaryons of Lentinula edodes was analyzed.The results showed that 58 pairs of SSR primers,accounting for 55.8% of the designed primers,produce 298 polymorphic fragments,accounting for 69.3% of the total 430 amplified fragments.The genetic similarity among the tested stains was analyzed by using NTSYS-pc21 software,and the results found significant differences existed between the cultivated strain and the wild strain.However,the cultivars with high similarity coefficients exhibited close genetic relationships between each other.At the same time,the study also showed that very close genetic relationship exists between the two protoplast mononuclear strains obtained from the same two nucleosomes.
出处
《发酵科技通讯》
CAS
2017年第4期233-237,共5页
Bulletin of Fermentation Science and Technology
基金
上海市科委重点科技攻关项目(10391900900)
上海市农委种业专项(沪农科种字(2012)第6号资助)
关键词
香菇
原生质体单核体
SSR标记
遗传多样性
Lentinula edodes
protoplasted monokaryon
SSR marker
genetic diversity
