摘要
以色氨酸生产菌Escherichia coli TRTH为出发菌株,在30 L发酵罐上进行实验,在发酵10 h后随糖外源流加1 g/L的琥珀酸,以降低代谢抑制物质的积累量,提高L-色氨酸的产量.实验结果显示:外源流加1 g/L的琥珀酸发酵与未流加琥珀酸发酵相比,菌体生物量与L-色氨酸产量分别提高了5.4%和10.0%,乙酸积累量下降了9.5%,糖酸转化率提高了4.0%.这表明外源流加1 g/L琥珀酸发酵可以提高L-色氨酸的产量,降低乙酸的积累量,提高糖酸转化率.
Escherichia coli TRTH was taken as the starting strain for tryptophan production.The succinic acid was added with glucose after 10 h fermentation in the 30 L fermenter to reduce the accumulation of metabolic inhibitors and increase the L-tryptophan yield.When 1 g/L succinic acid was added,the biomass,L-tryptophan production and glucose conversion rate were increased by 5.4%,9.98% and 4%respectively,while the accumulation of acetic acid was decreased by 9.5%.The results suggests that the addition of 1 g/L succinic acid can increase the production of L-tryptophan,reduce the accumulation of the acetic acid and improve the glucose conversion rate.
出处
《发酵科技通讯》
CAS
2017年第4期238-242,共5页
Bulletin of Fermentation Science and Technology
基金
天津市科技支撑计划重点项目(14ZCZDSY00015)
天津市科技特派员项目(15JCTPJC57000)
关键词
L-色氨酸
琥珀酸
乙酸
糖酸转化率
L-tryptophan
succinic acid
acetic acid
glucose conversion rate
