黄秋葵醇提物对小鼠的抗肥胖作用及与基因SREBP-1、FAS的相关性

Anti-obesity Effects of Alcohol Extract from Abelmoschus esculentus Linn. and Its Correlation with Gene SREBP-1 and FAS

目的研究黄秋葵粗提物(HQK)对营养性肥胖小鼠的抗肥胖作用。方法将50只ICR雄性小鼠,随机分成5组:空白对照组、模型组、黄秋葵醇提物组(100、200、400 mg·kg^(-1)),除空白对照组,其他4组小鼠均采用高脂饲料喂养建立肥胖小鼠模型,灌胃给药4周后,眼球取血,脱颈处死,取肝脏组织、脂肪。检测各组小鼠血清甘油三酯(TG)、总胆固醇(TC)、瘦素(LEP)、脂联素(ADP)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)。取腹部脂肪经RT-PCR,观察小鼠体内肥胖基因SREBP-1、FAS mRNA表达变化。结果黄秋葵醇提物高剂量可以有效控制肥胖小鼠体重,有效抑制TC、TG、LDL-C、LEP和升高HDL-C、ADP(P<0.01,P<0.05),有效改善血脂紊乱等症状,调节脂肪组织分泌蛋白质,并可有效抑制SREBP-1和FAS mRNA基因表达,达到对肥胖小鼠的治疗作用。结论黄秋葵粗提物治疗小鼠肥胖与其抑制SREBP-1和FAS的表达有关。

OBJECTIVE To study the anti-obesity effect of crude extract of okra on nutrition-obese mice. METHODS The fifty SPF ICR male mice were randomly divided into five groups：control（0.9% normal saline） , model（0.9% normal saline） , okra alcohol extraction high, medium and low dose group（400, 200, 100 mg · kg-1 ）. In addition to the mice in control group, the mice in other four groups were given high fat diet to make the model of obese mice, after oral administration for 4 weeks, eyeball blood of the mice was taken to measure the contents of serum triglyceride （ TG）, total cholesterol（ TC ）, leptin （ LEP）, adiponectin （ ADP）, high density lipoprotein（ HDL-C）, and low density lipoprotein（LDL-C）. Then mice were sacrificed to take their liver tissue and fat to detect the ex- pression of SREBP-1 and FAS mRNA by RT-PCR. RESULTS Compared with the model, the okra alcohol extract could effectively decrease the contents of TC, TG, LDL-C and LEP, increase the contents of HDL-C and ADP（P 〈 0.01,P 〈 0. 05 ）, improve the dys- lipidemia, and regulate the secretion of protein in adipose tissue, to achieve the therapeutic effect on obese mice. The expressions of SREBP-1 and FAS mRNA were also significantly inhibited by crude extract of okra. CONCLUSION The results show that treatment of crude extract of okra on obese mice is associated with the expressions of SREBP-1 and FAS.