八味龙钻颗粒对佐剂性关节炎大鼠的影响

Effects of Bawei Longzuan Granules in rats with adjuvant arthritis

目的研究八味龙钻颗粒对佐剂性关节炎大鼠的影响。方法将60只大鼠随机分为空白对照组、模型组、雷公藤多苷片组和八味龙钻颗粒低、中、高剂量组,除空白对照组外,其余各组均按每只0.1 mL弗氏完全佐剂(FCA)注射于大鼠左后足跖内。实验第8天开始灌胃给药,每日1次,给药体积10 mL/kg,八味龙钻颗粒剂量为5.9、11.8、23.6 g/kg,雷公藤多苷片为0.009 45 g/kg,空白对照组及模型组予等体积蒸馏水,连续灌胃给药21 d。观察佐剂性关节炎模型大鼠踝关节肿胀度及病理改变情况,并采用酶联免疫吸附测定(ELISA)法检测大鼠血清及关节组织匀浆中白细胞介素-1β(IL-1β)、肿瘤坏死因子α(TNF-α)的含量。结果与空白对照组比较,造模8d起模型组及各给药组大鼠足趾厚度显著增加,差异有统计学意义(P<0.01或P<0.05);与模型组比较,造模29 d八味龙钻颗粒高剂量组足趾肿胀度明显降低,差异有统计学意义(P<0.05);病理检测显示,八味龙钻颗粒高剂量组滑膜细胞增生程度、炎症细胞浸润程度均减轻;八味龙钻颗粒各剂量组血清IL-1β、TNF-α含量与模型组比较差异无统计学意义(P>0.05)。结论八味龙钻颗粒能降低佐剂性关节炎大鼠的足趾肿胀度,减轻滑膜细胞增生及炎症细胞浸润,对大鼠佐剂性关节炎病理模型具有一定的改善作用。

Objective To study the effects of Bawei Longzuan Granules in rats with adjuvant arthritis. Methods Sixty SD rats were randomly divided into blank control group, model group, Tripterygium Wilfordii Tablets group, and low, medium, high dose of Bawei Longzuan Granules group. Except for blank control group, the other groups were only given 0.1 mL of Freund complete adjuvant （FCA） injection into the left-hind feet of each rat. At the eighth day of the experiment, intragastric administration was started, once a day, the dosing volume was 10 mL/kg, the dosages of Bawei Longzuan Granules were 5.9, 11.8, 23.6 g/kg, the dosage of Tripterygium Wilfordii Tablets was 0.009 45 g/kg, blank control group and model group were given same volume of distilled water, continuous intragastric administration for 21 days. The conditions of ankle joint swelling and pathologic changes in model rats with adjuvant arthritis were observed, and enzyme-linked immunosorbent assay （ELISA） was used to detect the contents of interleukin-1β （IL-1β）, tumor necrosis factor α （TNF-α） in serum and joint tissue homogenate of rats. Results Compared with blank control group, started from the eighth day of making model, the feet thickness of model group and all medication groups was increased, the differences were statistically significant （P 〈 0.01 or P 〈 0.05）; compared with model group, the swelling degree of toes in high dose of Bawei Longzuan Granules group at the 29th day of modeling was decreased, the difference was statistically significant （P 〈 0.05）; the pathological examination showed that the proliferative degree of synovial cells and the invasive degree of inflammatory cells in high dose of Bawei Longzuan Granules group were all reduced; there were no statistically significant differences of the contents of serum IL-1β, TNF-α in each dose of Bawei Longzuan Granules group compared with those of model group （P 〉 0.05）. Conclusion Bawei Longzuan Granules can decrease the swelling degree of toes in rats with adjuvant arthritis, reduce the proliferation of synovial cells and inflammatory cell infiltration, which has certain improving effects for the pathological model of rats with adjuvant arthritis.