端粒酶和中心体微管蛋白α在宫颈鳞癌中的表达及临床意义

Expression and clinical significances of hTERT and α-Tubulin in cervical squamous cell carcinoma

目的探讨端粒酶和中心体微管蛋白α在宫颈鳞癌中的表达及临床意义。方法采用免疫组化法检测20例正常宫颈、40例宫颈上皮内瘤变和72例宫颈鳞癌组织中端粒酶和中心体微管蛋白α的表达,并分析其与临床病理特征的关系。结果在宫颈鳞癌组织中端粒酶和中心体微管蛋白α阳性表达率分别为84.72%、79.17%,高于正常宫颈组织和宫颈上皮内瘤变(P<0.05);端粒酶蛋白的表达与临床病理参数无关(P>0.05);中心体微管蛋白α的表达与患者病理分级、淋巴结转移有关(P<0.05),与其它临床病理参数无关(P>0.05);端粒酶和中心体微管蛋白α的表达呈显著正相关(r=0.353,P=0.002)。结论端粒酶和中心体微管蛋白α的表达在宫颈鳞癌的发生发展中起着重要作用,联合检测二者的表达,对早期诊断宫颈鳞癌具有积极的临床意义。

Objective To evaluate the expression of hTERT and α-Tubulin in cervical squamous cell carcinoma and its clinical significance. Methods The expression of hTERT and α-Tubulin in 20 specimen of normal cervical tissues, 40 specimen of CIN and 72 specimen of cervical squamous cell carcinoma were detected by immunohistochemical study, and their relations with clinical pathological features were analyzed. Results The positive expression rates of hTERT and α- Tubulin in cervical squamous cell carcinoma were 84.72% and 79.17%, respectively, which were higher than those in normal cervical tissues and CIN （P〈0.05）. hTERT expression were of no correlation with other clinical pathological parameters （P〉0.05）. α- Tubulin expression were significant correlation with clinical staging of tumor and lymph node metastasis （P〈0.05）, while they were of no correlation with other clinical pathological parameters （P〉0.05）. The expression of hTERT was in positive correlation to that of α-Tubulin （r=0. 353, P=0. 002）. Conclusion The expression of hTERT and α-Tubulin protein plays an important role in the development and progression of cervical squamous cell carcinoma. It is of great clinical significance for the early diagnosis of cervical squamous cell carcinoma.