秦艽不同配伍药对对风湿热痹类风湿性关节炎模型大鼠镇痛作用及血清TNF-α、IL-1β、PGE_2的影响

Effects of Qinjiao in Different Combinations on Content of Analgesia and Serum Contents of TNF-α,IL-1β and PGE_2 in Wind-Damp-Heat Arthralgia RA Model Rats

目的:研究秦艽不同配伍药对风湿热痹类风湿性关节炎(RA)模型大鼠镇痛抗炎作用的影响。方法:将80只SD大鼠随机分为空白组、模型组、病证模型组、阳性药组、单味秦艽组、秦艽威灵仙组、秦艽桑寄生组和秦艽防己组,采用Ⅱ型胶原诱导加"风湿热"环境因素刺激制备风湿热痹RA大鼠模型,连续18 d。造模结束后各给药组给予制备的对应药液灌胃(15 m L/kg),连续21 d。于给药前、给药后分别测量大鼠痛阈值、冷痛耐受时间及压力耐痛值,酶联免疫法测定各组大鼠血清中TNF-α、IL-1β、PGE2含量。结果:与病证模型组比较,秦艽配伍组大鼠痛阈值明显延长、冷痛耐受时间明显缩短、压力耐痛值明显升高,差异均具有统计学意义(P<0.01);且秦桑组痛阈值>秦防组>秦威组;秦防组冷痛耐受时间<秦桑组<秦威组;秦防组压力耐痛值明显高于秦桑组(P<0.01)。与空白组比较,模型组及病证模型组血清TNF-α、IL-1β、PGE2含量明显升高(P<0.01)。与病证模型组比较,秦防组TNF-α含量降低明显(P<0.01),阳性药组、秦桑组、秦威组次之(P<0.05);秦防组IL-1β含量降低明显(P<0.01),其余给药组IL-1β含量亦降低(P<0.05);秦威组、单味秦艽组秦防组PGE2含量降低明显(P<0.01),秦桑组次之(P<0.05)。结论:秦艽不同配伍药对风湿热痹RA大鼠均具有一定的镇痛抗炎作用,且平寒相配镇痛抗炎作用优于平温、平平相配,实验结果基本符合中医临床"疗热以寒药"的治疗原则,该配伍镇痛抗炎的作用机制与其能够减轻疼痛刺激、降低TNF-α、IL-1β、PGE2含量有关。

Objective： To observe effects of Qinjiao（ Gentianae macrophyllae Radix） in different combinations on the content of analgesia and the serum contents of tumor necrosis factor-α（ TNF-α）,interleukin-1β（ IL-1β） and prostaglandin E2（ PGE2） in wind-damp-heat arthralgia RA model rats. Methods： Eighty SPF SD rats were randomly divided into the blank group,collageⅡmodel group,wind-damp-heat arthralgia model group,Tripterygium group,single Qinjiao group,Qinjiao-Weilingxian（ Clematidis Radix et Rhizoma） group,Qinjiao-Sangjisheng（ Taxilli Herba） group,Qinjiao-Fangji（ Stephanlae Tetrandrae Radix） group. The wind-damp-heat arthralgia model was induced by collageⅡ in all groups except for the blank group and collageⅡmodel group,being exposed in wind-damp-heat environment for 18 consecutive days. After modeling,each administration group was gavaged with 15 m L/kg corresponding drug solution,once a day for 21 consecutive days. We measured the pain threshold,cold pain tolerance time and pressure pain tolerance value of rats before and after administration. The serum levels of TNF-α,IL-1β and PGE2 were detected. Results： Compared with the wind-damp-heat arthralgia model group,the pain threshold of Qinjiao compatibility groups rats was significantly prolonged,the cold pain tolerance significantly shorter and the pressure pain tolerance significantly increased. The difference was statistically significant（ P〈0. 01）. The pain threshold of Qin-Sang group Qin-Fang group Qin-Wei group. The cold pain tolerance of Qin-Fang group Qin-Sang group Qin-Wei group.The pressure pain tolerance of Qin-Fang group was significantly increased than that of the Qin-Sang group（ P〈0. 01）. Compared with the blank group,the TNF-α,IL-1β and PGE2 of serum in the collageⅡmodel group and wind-damp-heat arthralgia model group were significantly higher（ P〈0. 01）. Compared with the wind-damp-heat arthralgia model group,TNF-α of Qin-Fang group increased significantly（ P〈0. 01）,followed by Tripterygium group,Qin-Sang group,Qin-Wei group（ P〈0. 05）. IL-1β of Qin-Fang group increased significantly（ P〈0. 01）,followed by other treatment groups（ P〈0. 05）. The PGE2 of Qin-Wei group,single Qinjiao group and Qin-Fang group decreased significantly（ P〈0. 01）,followed Qin-Sang group（ P〈0. 05）. The analgesic and anti-inflammatory mechanism may be related to its ability,which can relieve pain stimuli,and decrease the content of TNF-α,IL-1β and PGE2. Conclusion： For the wind-damp-heat arthralgia RA,Qinjiao in different combinations have some effects of analgesia and anti-inflammatory,and the combined effect of mild and cold traditional Chinese medicine（ TCM） is better than the combined effect of mild and warm TCM or mild TCM drugs. Experimental results are consistent with the principles of traditional Chinese medicine clinical treatment of treating the hot diseases should use the cold medicine.