糖尿病肾阴虚证差异mRNA的筛选及分析

Different mRNAs in Type 2 Diabetes Mellitus within Kidney-Yin Deficiency

目的找出与糖尿病肾阴虚证发生、发展相关的mRNA,并对其可能参与的生物进程、信号通路进行分析,从基因层面探讨糖尿病肾阴虚发生、发展的分子机制。方法采用lncRNA/mRNA芯片进行基因表达谱检测,两组数据对比得到差异mRNA,再对差异mRNA进行GO分析、KEGG分析,从中找出可能与T2DM肾阴虚证的发病相关的基因及信号通路。结果筛选出差异mRNA 107条(FC≥2或≤0.5,p值≤0.05),其中上调88条,下调19条。具有显著性GO类18个,筛选到信号通路9条。结论糖尿病肾阴虚证的发生、发展过程存在炎症反应、细胞增殖等进程,可能与AMPK信号通路、补体和凝血级联反应信号通路有密切关系。

Objective The study try to screen out the different mRNAs related to type 2 diabetes mellitus within kidney - Yin de- ficiency. The possible biological processes and signaling pathways will be analysed. Meanwhile, the possible mechanism of the different mRNAs in type 2 diabetes mellitus within kidney - Yin deficiency would be discussed. Methods The lncRNAs and mR- NAs microarray is used in the study in order to get the expression of mRNAs. The data is compared to obtain the different mR- NAs. GO analysis and KEGG analysis will be performed on the differential mRNAs, in order to find out mRNAs and signaling pathways which were related to type 2 diabetes mellitus within kidney - Yin deficiency. Results In comparison of type 2 diabetes mellitus within kidney -Yin deficiency and healthy, the 107 different mRNAs are found. Among these mRNAs, the expression of 88 mRNAs is up -regulated, and 19 mRNAs is down -regulated. There are 18 classes of GO, 9 signaling pathways. Conclusion Inflammatory response and cell proliferation existence in type 2 diabetes mellitus within kidney - Yin deficiency. AMPK signaling pathway and complement and coagulation cascades may play an important role in type 2 diabetes mellitus within kidney - Yin defi- ciency.