脂多糖在创伤性颅脑损伤炎症反应中的作用

The role of LPS in inflammation after traumatic brain injury

目的研究分析脂多糖在创伤性颅脑损伤炎症反应中的作用。方法选取2010年9月至2014年9月间来本院就诊的创伤性颅脑损伤的患者36例进行回顾性研究,将所有患者根据格拉斯哥昏迷评分(GCS)分值分为轻度、中度、重度颅脑损伤组,每组12例患者,并且选取同期来本院进行体检的健康者12例作为对照组。在所有患者伤后1、2、3、5、7、14 d分别采集外周静脉血3 m L,分离获得血清。使用ELISA方法检测研究对象血清中TNF-α和IL-6水平。使用GREISS检测研究对象血清中NO水平。结果重度损伤组、中度损伤组患者1、2、3、5、7、14 d的血清中TNF-α和IL-6浓度与对照组比较差异均显著(P<0.05)。轻度损伤组患者1、2、3、5、7 d的血清中TNF-α和IL-6浓度与对照组比较差异均显著(P<0.05)。而轻度损伤组患者14 d的血清中TNF-α浓度和IL-6与对照组比较差异不显著(P>0.05)。重度损伤组患者1、2、3、5、7、14 d的血清中NO浓度与对照组比较差异均显著(P<0.05)。轻度损伤组、中度损伤组患者1、2、3、5、7 d的血清中NO浓度与对照组比较差异均显著(P<0.05)。而轻度损伤组、中度损伤组患者14 d的血清中NO与对照组比较差异均不显著(P>0.05)。结论脂多糖对创伤性颅脑损伤炎症反应中起到促进作用。

Objective The role of LPS in inflammation after traumatic brain injury was analyzed. Methods A total of 36 cases of traunatic brain injury patients treated in our hospital between September 2010 to September 2014 were studied retrospectively. All the patients were divided into mild, moterate, and severe traumatic brain injury groups according to Glasgow eorna scale （GC.S）, with 12 patients in each group. And 12 healthy eases who took medical examination in the hospital were selected as control group. All patients were collected 3 mL peripheral venous blood at 1 d, 2 d, 3 d, 5 d, 7 d, and 14 d after injury and serum was isolated. Serum TNF-α and IL-6 levels were detected by ELISA. Serum NO levels were detected by GREISS. Results TNF-α and IL-6 levels were significantly different between severe, moderate injury group and control group at 1 d, 2 d, 3 d, 5 d, and 7 d past-injury （P 〈0. 05）. TNF-α and IL-6 levels in mild injury group were significantly different from control group at 1 d, 2 d, 3 d, 5 d, and 7 d post-injury （P 〈0.05）. Serum TNF-α and IL-6 levels at 14 d post-injury between mild injury group and control group were not significantly different （P〉0.05）. Serum NO concentration of severe injury group at 1 d, 2 d, 3 d, 5 d, 7 d, and 14 d was significantly different from that of the control group （P 〈0.05）. There was significant difference in serum NO concentration at 1 d, 2 d, 3 d, 5 d, and 7 d post-injury between mild injury, moderate injury and control group （P 〈0.05）. But serum NO concentration in mild injury and moderate injury group at 14 d post- injury was not significantly different （P 〉0.05）. Conduslon lAX5 plays a catalytic role in the inflamrnatory response after traumatic brain injury.