三线定位法在大鼠心脏采血中的应用

Application of three-line positioning method in blood collection by cardiac puncture from rats

目的介绍一种改良的大鼠心脏穿刺采血方法。方法采用90只SPF级Wistar大鼠,雌雄各半,以10%水合氯醛溶液,按0.3 mL/kg体质量行腹腔注射麻醉,三线定位确定穿刺位点,使用采血针采血,利用采血管负压收集血液,记录每只大鼠采血量。结果以单只大鼠采血量达5 mL或以上为终末次采血成功计算成功率,90只大鼠共计采血90次,成功率约为82.2%,离心后血清总量约为260 mL,平均每只大鼠分离血清约为2.8 mL。结论改进后的心脏采血技术定位方法直观易学,且采血量多、血液质量高,同时创伤小、效率高,值得向广大同行推广应用。

Objective To introduce an improved method for collecting the blood of rat by cardiac puncture. Methods The study selected 90 Wistar rats, half male and half female, and then the rats were anesthetized by intraperitoneally injeeting 10% chloral hydrate solution according to 0.3 ml/kg body weight. Then using three-line positioning method to determine the puncture site,the blood collection needle and blood collection tube were used to collecting the blood, Finally,the blood volume of each rat was recorded. Results The success rate was calculated according to the single blood collection of 5 ml, or more, and our 90 rats were collected 90 times, the success rate was about 82.2%. After eentrifugation,the total serum was about 260 mL,the average serum separation of each rat was about 2.8 mL Conclusion The improved positioning method of heart blood collection is intuitive and easy to learn ,and the total volume and quality of blood is high,besides,the new method has a small surgical trauma and a high efficiency,which is worth to extend.