摘要
目的探讨槲皮素改善ADMA引起脐静脉内皮细胞凋亡的可能机制。方法体外培养人脐静脉内皮细胞,应用流式细胞仪技术检测细胞凋亡情况,应用Western blot法检测内质网应激蛋白PERK磷酸化水平及IRE1的蛋白表达量,用RTPCR检测ATF4及CHOP mRNA表达量。结果 ADMA诱导脐静脉内皮细胞凋亡,槲皮素可以抑制ADMA引起的PERK磷酸化及IRE1蛋白表达,并可以减少ATF4及CHOP mRNA量,继而改善ADMA引起的细胞凋亡。结论槲皮素抑制ADMA引起的脐静脉内皮细胞内质网应激继而抑制细胞凋亡。
Objective To study the effect of quercetin on ADMA induced endoplasmic reticulum stress responses (ER stress).Methods Human umbilical veins endothelial cells (HUVECs) were cultured, and left untreated or challenged for 24h with 100μmol/L ADMA in the absence and presence of 20μmol/L quercetin, the PERK and IRE1 was determined by Western blot. ATF4 and CHOP mRNA was detected by RT-PCR. Cells apoptosis was detected by flow cytometry.Results ADMA induced HUVECs apoptosis. Quercetin inhibited ADMA-induced PERK and IRE1 protein expression and ATF4 and CHOP mRNA expression. At last, Quercetin inhibited ADMA-induced cells apoptosis.Conclusion ADMA induced ER stress and HUVECs apotosis while quercetin inhibited ADMA-induced ER stress and apoptosis.
出处
《医学研究杂志》
2017年第11期34-37,共4页
Journal of Medical Research
基金
国家自然科学基金资助项目(81570660)
北京市优秀人才培养基金资助项目(2016000021469G223)