WWP2干扰对Huh7肝癌细胞的增殖、凋亡及细胞周期的影响研究

Effects of WWP2 Interference on Proliferation,Apoptosis and Cell Cycle of Hepatocellular Carcinoma Cell Huh7

目的探讨WWP2对Huh7肝癌细胞系的增殖、凋亡及细胞周期的影响研究。方法通过实时定量PCR(q PCR)、RNA干扰、流式细胞仪分析细胞周期及凋亡,了解WWP2在Huh7肝癌细胞周期的中作用。结果在肝癌组织中,WWP2 mRNA水平显著性表达;敲除细胞中的WWP2会抑制细胞增殖、使细胞停滞在G1期,并诱导细胞凋亡。结论 WWP2有可能通过调控细胞凋亡而促进肝癌细胞生长。

Objective To explore this effects of WWP2 interference on proliferation, apoptosis and cell cycle of hepatocellular carcinoma cell Huh7.Methods We used the experimental techniques of real-time quantitative PCR （qPCR）, RNA interference and flow cytometry to investigate the role of WWP2 in the cell cycle of hepatocellular carcinoma （Huh7）.Results WWP2 mRNA levels expressed significantly in hepatocellular carcinoma （HCC）. Knock except cells of WWP2 will inhibited cell proliferation, the cell arrest in the G1 phase, and induce cell apptosis.Conclusion WWP2 may promote the growth of hepatocellular carcinoma cells through the regulation of apoptosis in vitro.