6株猪源绿色气球菌的分离鉴定及耐药性分析

Isolation,identification and drug resistance analysis of 6 Aerococcus viridans from swine

从吉林省不同猪场病死猪肺脏组织中分离到6株优势菌群,通过细菌形态观察,生化试验及16SrDNA基因序列测定确定6株分离株为猪源绿色气球菌,将其命名为Aer-1~Are-6。动物致病性试验结果显示分离菌株对小鼠均有致病性。动物回归致病性试验表明,分离株对仔猪具有致病性。药敏试验结果显示,分离菌株具有多重耐药性,只对阿米卡星、氟苯尼考、环丙沙星及恩诺沙星较敏感。

Six Aerococcus viridans were collected by morphological characteristics,biochemical test and 16S rDNA sequencing from lung of pigs at different regions in Jilin province,we named it Are 1 to Are-6. Animal pathogenicity test showed that the isolated strains had pathogenicity to mice and piglet. Drug sensitive test results showed that the isolated strains presented multi drug resistanee（MDR） ,and were only sensitived to amikacin, florfenicol, ciprofloxacin and enrofloxacin.