摘要
为了明确持续低产蛋率开产蛋鸭群的主要病毒病感染情况,应用已建立的间接ELISA方法和血凝抑制试验对2013年1月至2015年10月采自福建、广东、广西、江西、浙江、江苏和安徽等7省(区)免疫过H5亚型和H9亚型禽流感灭活疫苗但未免疫禽1型副黏病毒病疫苗和禽坦布苏病毒病疫苗,表现为持续低产蛋率的29个开产蛋鸭群的4 737份血清样品进行抗体检测,同时采集蛋鸭卵巢组织进行RT-PCR检测。结果显示,29个开产蛋鸭群中,H5亚型禽流感、H9亚型禽流感、禽1型副黏病毒病和禽坦布苏病毒病抗体的群阳性率分别为100.0%,100.0%,10.3%和100.0%,群内阳性率分别为79.4%~100.0%,82.5%~100.0%,0.0%~13.1%和33.7%~100.0%;蛋鸭卵巢组织中H5亚型禽流感病毒、H9亚型禽流感病毒、禽1型副黏病毒和禽坦布苏病毒的阳性率依次为13.8%(4/29),0.0%(0/0),3.5%(1/29)和86.2%(25/29)。上述结果表明,我国以上7省(区)表现持续低产蛋率的开产蛋鸭群存在禽坦布苏病毒的严重感染,应引起养鸭生产者的高度重视。
In order to evaluate the primary virus infection in persistent low-productivity duck flocks,a total of 4737 serum samples and ovary samples(3 samples per flock) from 29 egg-laying duck flocks distributed in Fujian, Guangdong, Guangxi ,Jiangxi, Zhejiang ,Jiangsu, and Anhui provinces from January 2013 to October 2015 were collected for indireeted ELISA and RT-PCR detection,respectively. All flocks were immunized with H5 and H9 vaccines but no avian paramyxovirus type 1 and avian Tembusu virus vaccines, which presented persistently low laying rate. The results showed that the antibody positive rates in inter-flock of AIV-H5, AIV-H9, avian paramyxovirus type 1 ,and avian Tembusu virus infection were 100.0%, 100.0 %,10.3% ,and 100% ,respectively, those in intra-flock of 79. 4%-100. 0%, 82 .5%-100. 0%, 0.0%-13.1% and 33. 7%-100. 0%,respectively. Meanwhile,the flock positive rates of AIV-HS,AIV-H9,avian paramyxovirus type 1 and avian Tembusu virus detected by RT-PCR were 13.8%(4/29),0.0%(0/0) ,3.5%(1/29),and 86.2%(25/29), respectively. The above results proved that avian Tembusu virus infection was widely spread in egg-laying duck flocks and directly related to persistently low-yield in duck flocks.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2017年第12期2288-2293,2299,共7页
Chinese Journal of Veterinary Science
基金
现代农业产业技术体系建设专项资助项目(CARS-43)
国家自然科学基金资助项目(31472222
30972203)
福建省属公益类科研院所基本科研专项资助项目(2014R1023-3
2016R1022-1
2016R1022-9)
福建省自然科学基金资助项目(2015J01113)
福建省优良蛋鸭品种与设施化养殖创新产业化工程资助项目(2014-2016)
福建省畜禽疫病防控技术重大研发平台子专题资助项目(2014N2003-8)
福建省农业科学院青年基金资助项目(2014CX-22)