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2株枯草芽孢杆菌协同降解黄曲霉毒素B_1的效果 被引量:2

Co-degradation effect on aflatoxin B_1 by two strains of Bacillus subtitles
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摘要 旨在研究2株枯草芽孢杆菌协同降解黄曲霉毒B1(AFB1)的作用。将2株枯草芽孢杆菌进行生理生化特性鉴定和16SrRNA序列测定后,以AFB1降解率为依据,分别进行优化培养并观察协同降解作用。结果表明:菌株K-3优化条件为装液量50mL/250mL、温度37℃、4%接种量、初始pH7.0、发酵周期48h,150r/min振荡培养,对AFB1降解率为88.07%(P<0.05);菌株K-TM装液量为50mL/250mL、温度37℃、6%接种量、初始pH7.0、发酵周期60h,150r/min振荡培养,对AFB1降解率为84.81%(P<0.05);K-3、K-TM按体积1∶2组合,对AFB1协同降解率达到88.43%。本研究为益生菌在降解黄曲霉毒素中的应用奠定理论基础及试验依据。 This experiment was conduced to investigate the co-operation effect on aflatoxin B1 (AFB1) by two strains Bacillus" subtitles. The physiological and biochemical characteristics,and 16S rRNA sequence were determinated in two strains of Bacillus substilis, based on degradation rate of AFB1 , cultivation and co-degradation were optimized. The obtained best fermentation condition was described below: (K-3) amount of load 50 mL/250 mL,optimum temperature 37℃ ,amount of inoculation 4% ,original pH7.0,and 150 r/rain for 48 h,degradation rate reached by 88.07% (P〈0.05). (K-TM) amount of load 50 mL/250 mL,optimum temperature 37℃ ,amount of inoculation 6%,original pH7.0,and 150 r/rain for 60 h,degradation rate reached by 84.81%(P〈0.05). The optimal proportion of strain K-3 and strain K-TM was 1 : 2 and the maximum AFB1 degradation rate was 88.43 %. This study will provide a preliminary fundation for the future application of probiotics in the biological degradation of aflatoxin(AFT).
出处 《中国兽医学报》 CAS CSCD 北大核心 2017年第12期2397-2401,共5页 Chinese Journal of Veterinary Science
基金 河南省科技攻关资助项目(142102310034) 河南省高等学校青年骨干教师计划资助项目(2014GGJS-124) 河南牧业经济学院科技创新团队资助项目(HUAHE2005001)
关键词 枯草芽孢杆菌 黄曲霉毒素B1 降解 Bacillus subtitles aflatoxin B1 degradation
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