香薷水煎液HPLC指纹图谱

HPLC Fingerprint of Water Decoction of Moslae Herba

目的:为全面控制香薷药材的质量,建立香薷水煎液的HPLC指纹图谱,更为新加香薷饮成品颗粒剂的质量控制奠定基础。方法:测定10批不同产地香薷药材水煎液的HPLC指纹图谱,色谱条件为采用Phenomenex Luna C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1%磷酸水,梯度洗脱,柱温40℃,流速1 m L·min^(-1),检测波长269 nm。将10批指纹图谱导入"中药色谱指纹图谱相似度评价系统"(2012.130723版),进行色谱峰匹配,以咖啡酸峰为参照峰,采用中位数法生成对照图谱,并对10批指纹图谱进行相似度评价。结果:建立了香薷药材水煎液的指纹图谱,标定出14个共有峰;对10批不同产地香薷药材水煎液的指纹图谱进行相似度评价,相似度均达到0.90以上;由共有峰的相对峰面积结果分析,不同产地香薷药材的质量存在较小差异。结论:该研究方法重复性好,可行性强,为香薷的全面质量评价奠定了基础,更为新加香薷饮质量标准的拟订提供了参考。

Objective： To establish an HPLC fingerprint of water decoction of Moslae Herba for quality evaluation, and lay the foundation for the quality control of Xinjia Xiangruyin granules. Method： The chromatographic fingerprints of 10 batches of water decoction of Moslae Herba from different places of origin were obtained on Phenomenex Luna C_（18）（ 4. 6 mm × 250 mm,5 μm）,with acetonitrile-0. 1% phosphoric acid solution as the mobile phase for gradient elution. The column temperature was maintained at 40 ℃ at a flow rate of1 m L·min^-1,and the detection wavelength was 269 nm. 10 batches of fingerprints were imported into Similarity Evaluation System for Chromatographic Fingerprint of TCM（ 2012. 130723） for chromatographic peak matching.With caffeic acid as the reference peak,the midpoint method was used to establish the reference fingerprint,which was then compared with the fingerprint of 10 batches of Moslae Herba. Result： The HPLC fingerprint of Moslae Herba showed 14 common peaks. The similarities of 10 peaks from different origins were all more than 0. 90. Then the analysis results on relative peak areas,showed less differences in the quality of Moslae Herba from 10 origins.Conclusion： With good reproducibility and feasibility,this method can be used as the means to assess the quality of Moslae Herba and provides reference for preparing the quality standard of Xinjia Xiangruyin granules.