摘要
为了测定去滞散中枳壳的含量,采用C18柱(4.6 mm×150 mm,5μm),在室温下,以乙腈-水(20∶80,用磷酸调节pH值至3)为流动相,283 nm为检测波长,流速为1.0 m L/min,建立了反相高效液相色谱(RP-HPLC)测定方法。结果显示,柚皮苷在0.08~4μg范围内线性关系良好,R^2=0.9999,回收率为94.4%~101.7%,RSD为2.23%;新橙皮苷在0.08~4μg范围内线性关系良好,R^2=0.9995,回收率为90.6%~100.0%,RSD为2.85%。本方法简便、准确、快速,可用于控制去滞散中枳壳的含量。
A method for the determination of Aurantii fructus in Quzhi San was developed by the reverse highperformance liquid chromatography （RP -HPLC）. The C18 colum （ 4. 6 mm× 150 mm, 5 μm） was used withacetonitrle-water（20 ∶ 80, pH was adjusted to 3 by H3PO4） and the detection wavelength was 283nm.The resultsshowed that Naringin had a good linear relationship at the range of 0.08～4 μg（R2= 0.9999）and the recovery ratewere 94.4%～ 101.7%,RSD = 2.23%. Neohesperidin had a good linear relationship at the range of 0.08 ～ 4 μg（R2= 0.9995）and the recovery rate were 90.6%～100.0%,RSD= 2.85 %. The method was simple, accurate andfast in the determination of Aurantii fructus in Quzhi San.
出处
《中国兽药杂志》
北大核心
2017年第12期36-41,共6页
Chinese Journal of Veterinary Drug
基金
公益性行业(农业)科研专项(项目编号:20130340-15)
