基于D-loop和Cyt b序列的大海马遗传变异分析

Analysis of Genetic Variation of Hippocampus kuda Base on D-loop and Cyt b Sequences

[目的]对大海马D-loop和Cyt b序列进行遗传变异分析。[方法]基于D-loop和Cyt b部分序列对东山岛(20尾)和泉州市(20尾)养殖场大海马进行遗传变异分析。[结果]获得的D-loop和Cyt b序列分别为707~709 bp和404 bp,且2种序列在2个大海马群体之间极其保守。D-loop和Cyt b序列G+C含量明显低于A+T含量,C含量也低于G含量,Cyt b序列第3位密码子G含量最少。东山岛和泉州市的大海马(各20条)D-loop序列的平均核苷酸差异数分别为0.489和0.958,且分别定义了3种和6种单倍型,东山岛和泉州市的大海马(各20条)Cyt b序列在2个群体之间相似度为100%,只定义了1种单倍型。[结论]研究表明D-loop和Cyt b序列在2个大海马群体中变异较小。

[ Objective]To study the genetic variation of Hippocampus k u d a base on D-loop and Cyt 6 sequences. [Method]In this paper,the genetic variations of H. k uda between Dongshan island（20 individuals） and Quanzhou City（20 individuals） were loop and Cyt 6 sequences. [ Result] D-loop and Cyt 6 sequences were 707 -709 bp and highly conserved between the two populations. G ＋ C content of D-loop and Cyt 6 sequences were significantly lower than A ＋ T, however, the C content was lower than G, and G content of Cyt 6 sequences were the least in the third codon. The average number of nucleotide differences of 20 D-loop sequences of H. k u d a from Dongshan island and Quanzhou City were 0.489 and 0. 958,and 3 and 6 kinns of haplotypes were de-fined respectively,meanwhile,the similarity of Cyt 6 sequences were 100% with a total of 1 haplotype between the two groups. [ Conclusion] Al the results showed that the variation of D-loop and Cyt 6 sequences were relatively little in the two population of H.kuda.