Roux-en-Y胃旁路术对大鼠肠道厚壁菌群及APPL1的影响

Effects of Roux-en-Y Gastric Bypass on Intestinal Thickening Bacteria and APPL1 in Rats

目的探讨Roux-en-Y胃旁路术对肠道厚壁菌群及APPL1的影响.方法使用Roux-en-Y胃旁路术作用于肥胖大鼠模型,并观察术后以及术后粪菌移植对大鼠相关代谢指标的影响,使用Western blot及q PCR检测不同分组的厚壁菌群及APPL1的表达.结果手术后大鼠的体重较术前明显下降(P<0.05),粪菌移植后体重有所回升,S+T组的体重较S组明显升高(P<0.01);手术后大鼠的血浆中TG表达较术前明显下降(P<0.01),S+T组的血浆中TG表达较S组明显上升(P<0.05);同时在骨骼肌中发现S组的TG表达明显低于C组,在S+T组中的TG表达明显高于S组(P<0.05);同时发现,在S组中TCH的表达明显较C组低,而S+T组的TCH表达较S组升高(P<0.05);FINS在S组中的表达较C组明显下降,FINS在S+T组的表达较S组明显升高(P<0.05);FGB在S组中的表达较C组明显下降,S+T组的FGB表达较S组明显上升(P<0.05);ISI在S组的表达较C组明显上升(P<0.05),ISI在S+T组的表达组较S组明显下降(P<0.01).厚壁菌群在S的表达较C组明显降低(P<0.05).Western blot检测以及m RNA的检测发现APPL1在S组的表达较C组明显升高(P<0.01),S+T组中APPL1的表达较S组明显降低(P<0.05).结论 Roux-en-Y胃旁路术可能是通过影响厚壁菌群的表达量进而调节体重的.

Objective To investigate the effect of Roux-en-Y gastric bypass on intestinal thick-walled bacteria and APPL1.Methods The effect of postoperative and postoperative fecal transplantation on the metabolic index of rats was observed by Roux-en-Y gastric bypass.The thick-walled bacteria and APPL1 expression were detected by Western blot and qPCR.Results The body weight of the rats in the S＋T group was significantly higher than that in the S group（P〈0.05）.The body weight of the rats in the S＋T group was significantly higher than that in the control group（P〈0.05） The expression of TG in S＋T group was significantly higher than that in S group（P〈0.05）.The expression of TG in S group was significantly lower than that in group C（P〈0.05） The expression of TCH in the T group was significantly higher than that in the S group（P〈0.05）.The expression of TCH in the S group was significantly higher than that in the S group（P〈0.05） The expression of FINS in S group was significantly higher than that in group C（P〈0.05）.The expression of FINS in S group was significantly higher than that in group S（P〈0.05）.The expression of ISI in S group was significantly higher than that in group S（P〈0.05）.The expression of ISI in S group was significantly higher than that in group S（P〈0.05）.The expression of S thick-walled bacteria in S was significantly lower than that in group C（P〈0.05）.Western blot and mRNA detection showed that the expression of APPL1 in S group was significantly higher than that in group C（P〈0.01）.The expression of APPL1 in S＋T group was significantly lower than that in S group（P〈0.05）.Conclus ion Roux-en-Y gastric bypass may be mediated by affecting the expression of thick-walled flora.