番鸭A-FABP基因单核苷酸多态与生产性状的相关性研究

SNP of A-FABP Gene Relating to Meat Productivity of Muscovy Ducks

为了解A-FABP基因单核苷酸多态在番鸭群体中的分布情况,以及单核苷酸多态与生产性能的关联性,采用PCR-SSCP技术对番鸭A-FABP基因进行多态性研究,分析该基因与体重、屠宰性能、常规肉品质的相关性。结果表明:番鸭A-FABP基因上发现A-C突变,位于第2内含子第201处碱基,形成了AA、AB、BB3种基因型,A等位基因频率为0.8241,B等位基因频率为0.175 9,群体纯合度为0.710 0,多态性信息含量为0.247 9。除第2、4周龄外,AA型公鸭早期体重显著高于AB型公鸭,母鸭不同基因型之间无显著差异。公鸭的宰前活重、屠体重、半净膛重、全净膛重、胸肌重、腹脂重、腹脂率指标在不同基因型之间存在显著性差异,AB型均小于AA型。母鸭只有腹脂重、腹脂率在不同基因型间存在显著性差异,AB型低于AA型。番鸭AFABP基因第2内含子A201C单核苷酸突变对腹脂沉积、公鸭体重有显著影响。

The distribution of single nucleotides in the A-FABP gene of Muscovy ducks was believed to closely related to the meat productivity of the duck.This study applied PCR-SSCP to unveil the single nucleotide polymorphisms(SNPs)of the ducks that were genetically varied.Then,these SNPs were corrected to the body weight,slaughtering property and meat quality of the ducks.The results showed that the SNP of A-C was at the 201^(st) site in the intron 2 of the A-FABPgene,and the mutation formed the genotypes,AA,AB and BB.The allele frequencies of A and B were determined to be 0.824 1 and 0.175 9,respectively.The homozygosity was 0.710 0 in the duck population;and,the polymorphic information content(PIC),0.247 9.Other than the 2-and 4-week-old ducks,the individual weight of AA males was significantly higher than that of AB males,and no significant difference on the female ducks.The weights of live bird,slaughtered carcass,dressed carcass,half-eviscerated carcass,eviscerated carcass,breast meat,and abdominal fat,as well as the percentage of abdominal fat of a male duck differed significantly depending upon the genotypes,as those of the AB genotype being lower than those of the AA genotype.For the female ducks,only the weight and percentage of abdominal fat were significantly lower in the AB than AA genotype.The SNP of A201 Cin the intron 2 of the A-FABP gene played an important role on the abdominal fat deposit and body weight of a male Muscovy duck.