绣球菌瓣片与基部多糖的分子量分布比较

Molecular Weight Distributions of Polysaccharides in Flabella and Roots of Sparassis latifolia Fruiting Bodies

为了明确广叶绣球菌Sparassis latifolia子实体的瓣片、基部可溶性多糖分子量分布差异,将新鲜绣球菌子实体经冷冻干燥、热水提取得到绣球菌瓣片多糖和基部多糖,通过β-葡聚糖酶酶解试验和高效凝胶渗透色谱(HPSEC)对绣球菌瓣片、基部多糖的分子量分布规律进行研究。结果表明:广叶绣球菌的瓣片多糖与基部多糖的重均分子量和分子量分布显著不同,绣球菌的瓣片多糖的重均分子量为3.88×10~5 Da,主要成分的重均分子量为4.30×10~5 Da;而基部多糖的重均分子量为1.43×10~6 Da,主要由2.85×10~4 Da的小分子量部分和分子量大于2×10~6 Da的大分子量部分构成。酶解试验结果表明绣球菌基部多糖的水溶液中分子量大于2×10~6 Da的部分无法被β-葡聚糖酶酶解。

Distributions of molecular weight(Mw)of soluble polysaccharides in the flabella and roots on fruiting bodies of Sparassis latifolia were compared.The fruiting bodies were freeze-dried after harvest prior to a hot-water extraction to collect polysaccharides for a high performance size exclusion chromatography(HPSEC)analysis and aβ-glucanase digestion test.The HPSECresults showed that the average Mws and Mw distributions of polysaccharides significantly differed in the flabella and the roots.The polysaccharide Mw in the flabella was 3.88×10~5 Da on average with a main component at 4.30×10~5 Da;whereas,that in the roots was 1.43×10~6 Da with a majority of 2.85×10~4 Da and a few largerthan 2×10~6 Damolecules.The enzymatic digestion test indicated thatβ-glucanase could not degrade polysaccharides in the fruiting bodieswith a molecular weight greater than 2×10~6 Da.