罗非鱼养殖池塘水和表层沉积物中蓝藻的群落结构及影响因素研究

Community structure of cyanobacteria in water and surface sediment of tilapia ponds and the influencing factors

为了研究罗非鱼养殖池塘水和表层沉积物中蓝藻(蓝细菌)的群落结构、多样性状况和影响因素,采用高通量测序技术平台对细菌16S rRNA基因进行测序,对其中的蓝藻组成进行分析。结果表明:水和表层沉积物中的蓝藻群落结构存在显著差异,但其中的优势目是相同的,为聚球藻目、Cyanobacteria_norank和色球藻目;水中的蓝藻群落结构受到月份变化的影响比较大,并且与系统中氮净输入量有关。表层沉积物中的蓝藻群落受到池塘差异的影响更大,且与系统中磷净输入量有关。试验条件下,罗非鱼对系统中蓝藻群落多样性的影响主要来自于直接或间接的营养物质输入。

In order to investigate the community structure and diversity of cyanobacteria in water and sediment of tilapia ponds,the Illumina high-throughput sequencing and the bioinformatics analysis were performed.The results showed that there were significant differences of cyanobacteria community structure between water and surface sediment samples.Nonetheless,the dominant groups in these two habitats were the same,which were Synechococcales,Cyanobacterianorank and Chroococcales.Further researches indicated that the cyanobacteria community in water was affected by the monthly variations,and was closely related to the net input of nitrogen to the ponds systems.However,the cyanobacteria community in surface sediment was more affected by the ponds variations and was related to the net input of phosphorus to the ponds systems.To sum up,the tilapia cultivations in the ponds systems affected the cyanobacteria communities in water and surface sediment more by the way of nutrients input than by direct feeding.