中华绒螯蟹成熟雄体生殖系统的生化组成分析

Biochemical composition in the reproductive system of the mature male Chinese mitten crab,Eriocheir sinensis

中华绒螯蟹雄性生殖系统较为复杂,尚未见其各组成部分生化成分的全面分析,本实验探究了成熟雄蟹生殖系统各部分的组织系数、常规生化成分、脂肪酸组成和氨基酸含量。结果显示:(1)副性腺指数(AGI)显著高于精巢指数(TI)和输精管指数(VDI),P<0.05。(2)就常规生化成分而言,精巢中的水分、总脂和总碳水化合物含量均显著高于输精管和副性腺(P<0.05),而粗蛋白含量则以副性腺最高,输精管次之,精巢最低(P<0.05)。(3)就脂肪酸组成而言,生殖系统各部分的总饱和脂肪酸(ΣSFA)、n-3多不饱和脂肪酸总量(∑n-3PUFA)和n-3/n-6均无显著差异(P>0.05),但精巢中的C20:3n3、C20:4n6、C22:6n3、总多不饱和脂肪酸(∑PUFA)、∑n-6PUFA和总高度不饱和脂肪酸(∑HUFA)含量显著高于其他两个部位(P<0.05)。(4)就氨基酸组成而言,输精管中赖氨酸(Lys)、缬氨酸(Val)和组氨酸(His)含量最高,副性腺次之,精巢最低(P<0.05),副性腺中其余大部分的氨基酸及总氨基酸含量最高。综上,中华绒螯蟹成熟雄体精巢中主要积累脂肪、碳水化合物、PUFA和HUFA,而副性腺中粗蛋白和大部分氨基酸含量较高,这可能与其不同的生理功能有关。

The reproductive system of Chinese mitten crab,Eriocheir sinensis is complicated,and accessory gland,testis and vas deferens are three major constituents.However,no available information could be found on the biochemical composition of these three tissues for the mature male E.sinensis.Therefore,this study was conducted to investigate the proportion,proximate composition,fatty acid composition and amino acid contents in the reproductive system of mature male E.sinensis.The results showed that：（1） the accessory gland index（AGI） was significantly higher compared to the testis index（TI） and vas deferens index（VDI）（P〈0.05）.（2） For the biochemical composition,the contents of moisture,total lipid and total carbohydrate in the testis were significantly higher than those of the other parts（P〈0.05）,while the highest crude protein content was detected in the accessory gland（P〈0.05）.（3） For fatty acids profile,no significant differences were found in the percentages of the most saturated fatty acid（SFA）,total saturated fatty acid（ΣSFA） and monounsaturated fatty acid（MUFA） among three parts,while the testis had the highest levels of C20∶3n3,C20∶4n6,C22∶6n3,total polyunsaturated fatty acids（∑PUFA）,∑n-6 PUFA and total highly unsaturated fatty acids（∑ HUFA）（P〈0.05）.（4） For amino acids composition,the highest contents of the major amino acids were detected in accessory gland,while the contents of L-lysine（Lys）,valine（Val） and Histidine（His） were shown as VD〉AG〉T（P〈0.05）.In conclusion,the high contents of total lipid,total carbohydrate,PUFA and HUFA were stored in the testis of mature E.sinensis,while the accessory gland had the highest contents of crude protein and total amino acids.These differences may indicate their different physiological function for these tissues of mature E.sinensis.