绵羊痘病毒的检测与遗传进化分析

Detection and Phylogenetic Analysis of Sheep Poxvirus

羊痘是全球最严重的动物传染性痘病。近年来,羊痘病毒在非洲北部和中部、中东、亚洲大部分地区广泛流行,给养殖业带来巨大的经济损失。论文对4只疑似羊痘病毒感染绵羊进行诊断,通过临床观察,病理剖检,病理组织学观察,实验室检测,确定该病为绵羊痘,并对分离的病毒主要基因进行PCR扩增及遗传进化分析,发现此毒株属于亚洲系,且我国流行株基因比较稳定,变异不大。这不仅为绵羊痘病的诊断提供了技术手段,还丰富了我国羊痘病毒感染的流行病学资料,为进一步研究羊痘病毒的变异等提供数据支持。

Capripox virus （CaPV） is one of the most concerned infectious diseases around the world.In recent years,CaPV circulated in North and Middle Africa, Middle East and Asia, which have caused great damage to the breeding industry. In this research, four sheep were preliminarily speculated to be infected with CaPV.A final diagnosis was made based on clinical signs,postmortem examinations,histopathological analysis,and PCR detection.Phylogenetic analysis indicated that the causative CaPV belongs to Asia-lineage,and they are few genetic mutation in China.Our results provided useful information for the diagnosis of capripox.Additionally,the research is valuable for the analysis of virus evolution and mutation.