IL-6-174G>C基因多态性与南京地区汉族人群冠心病的相关性

Association of interleukin-6 gene-174G > C polymorphisman with coronary heart disease of Chinese Han population in Nanjing area

目的探讨IL-6基因启动子区域-174G>C基因多态性与南京汉族人群冠心病(CHD)的关系。方法聚合酶链-限制性片段多态性(PCR-RFLP)检测235例CHD患者和175例体检健康者,分析各组IL-6-174G>C基因多态性,并进行Hardy-Weinberg平衡检验,选取部分DNA样本进行基因测序,以验证其基因型。同时检测各组血脂、Glu、C反应蛋白、糖化血红蛋白的表达水平。结果 CHD组和健康人对照组GG、GC、CC基因型频率分别为98.7%、1.3%、0和97.7%、2.3%、0,G、C等位基因频率分别为99.4%、0.6%和98.9%、1.1%,基因型和等位基因频率在两组间的分布差异无统计学意义(P均>0.05),与健康人对照组相比,CHD组吸烟、SBP、DBP、TG、CHOL、HDL-C、Apo A-I、Apo B、CRP、Hb A1c差异均有统计学意义(P均<0.05),而年龄、性别、Glu差异均无统计学意义(P均>0.05)。结论 IL-6-174G>C基因多态性与南京地区汉族人群CHD没有明显的相关性。

Objective To investigate the relationship of -174G 〉 C polymorphism of interleukin 6 （IL-6） gene promoter region up- stream-with coronary heart disease （CHD） of Chinese Han population in Nanjing area. Methods The polymorphism of IL-6-17gG 〉 C gene was confirmed in 235 patients with CHD and 175 healthy individuals by PCR-RFLP, and Hardy-Weinberg equilibrium was test- ed. DNA samples were selected for sequencing to verify their genotype. The concentration of blood sugar, lipid, C reaction protein （CRP） and glycosylated hemoglobin （HbA1c） in the samples were measured simultaneously. Results The genotype distributions of GG, GC and CC were 98.7%, 1.3% and 0 in CHD group and 97.7%, 2.3% and 0 in control group, respectively. The frequencies of G and C alleles were 99.4%, 0.6% and 98.9%, 1.1% in the two groups. There were no statistical significance for frequencies of genotype and alleles between the two groups （ all P 〉 0.05 ）. Compared with the control group, the differences of smoking, systolic blood pressure （SBP）, diastolic blood pressure （DBP）, triglyceride （TG）, cholesterol （CHOL）, low-density lipoproteins-cholesterol （ LDL-C）, high-density lipoproteins-cholesterol （ HDL-C ）, apolipoprotein A-I （ ApoA-I）, ApoB, CRP and HbA1 c were statistically significant （all P 〈 0.05）, while age, sex and blood glucose were not statistically significant （all P 〉 0.05）. Conclusion IL-6-174 G 〉 C gene polymorphism should not remarkably correlated with CHD in Chinese Han population in Nanjing area.