川崎病患儿血浆sFKN、TNF-α的变化以及与冠状动脉损伤的关系

Changes of plasma sFKN,TNF-αand its relationship with coronary artery lesions in children with kawasaki disease

目的探讨可溶型不规则趋化因子(sFKN)和肿瘤坏死因子-α(TNF-α)在川崎病(KD)患儿血浆中的变化以及与冠状动脉损伤的关系。方法选取2015年6月~2016年12月于我院儿科住院的KD患儿38例,根据心脏超声心动图检查结果分为冠状动脉损伤(CAL)组16例和无冠状动脉损伤(NCAL)组22例;随机选取同期门诊健康体检儿童20例作为对照组。采用酶联免疫吸附法(ELISA)测定各组儿童血浆sFKN、TNF-α水平。结果 KD患儿急性期血浆sFKN、TNF-α水平高于对照组,而亚急性期较急性期则显著降低。KD患儿急性期CAL组血浆sFKN、TNF-α的水平高于NCAL组,亚急性期CAL组仍较NCAL组高,但差异无统计学意义(P>0.05)。相关性分析显示,sFKN与TNF-α在KD患儿急性期成正相关关系。结论 sFKN和TNF-α共同参与了KD血管炎及冠状动脉损伤的形成过程。

Objective To investigate the changes of plasma soluble fractalkine（sFKN）,tumor necrosis factor-α（TNF-α）levels and its potential role of coronary artery lesions of patients with Kawasaki disease（KD）.Methods Thirty-eight inpatient children with KD in department of pediatrics of our hospital from June 2015 to Dec.2016 were collected and divided into two groups according to results of echocardiography：coronary artery lesion（CAL）group（n=16）and non-coronary artery lesion（NCAL）group（n=22）.Twenty healthy children in the same period were chosen as the control group.The levels of plasma sFKN and TNF-αwere measured by enzyme-linked immunosorbent assay（ELISA）respectively.Results Compared with the control groups,plasma sFKN and TNF-αlevels were significantly elevated in the acute phase of KD.In the subacute phase of KD,they were significantly lower.The plasma sFKN,TNF-αlevels of CAL group were higher than NCAL group in the acute phase of KD and they were still higher in the subacute phase of KD,but the difference was not statistically significant（P〉0.05）.The correlation analysis show that the plasma sFKN levels was positively correlated with TNF-αlevels in the acute phase of KD.Conclusions The plasma sFKN and TNF-αare potential pathological factors in the formation of KD vasculitis and coronary artery lesions.