惠州地区无偿献血人群人类嗜T淋巴细胞病毒感染情况调查

Investigation on human T lymphocyte virus infection among voluntary blood donors in Huizhou area

目的通过调查惠州地区无偿献血人群中人类嗜T淋巴细胞白血病病毒(HTLV-Ⅰ/Ⅱ)的感染状况,初步了解其在人群中的流行特点,为本地区的用血安全提供合适的策略。方法采用双抗原夹心法ELISA试剂,对惠州地区2016年3月-2016年12月共56 128名献血者进行HTLV-Ⅰ/Ⅱ抗体初筛,初筛阳性标本再剪血袋辫子一起双孔复查,复查阳性标本进行电化学发光法(ECL)和蛋白印迹法(WB)确证。结果 56 128份无偿献血者标本初筛阳性20份,确证实验5例阳性,阳性率为0.01%。5名确证阳性者皆为男性,其中3名ECL和WB均阳性。结论调查结果表明惠州地区献血人群中存在HTLV-Ⅰ/Ⅱ感染,从安全输血角度综合分析,有必要对献血者进行HTLV筛查。

Objective To assess the prevalence of human T cell leu-kemia virus-Ⅰ/Ⅱ（HTLV-Ⅰ/Ⅱ）infection among blood donors in Huizhou area, obtain information of the epidemic characteristics in the crowd, and provide the appropriate strategies for blood transfusion safety. Methods Double antigen sandwich ELISA kit were used to screen HTLV-Ⅰ/Ⅱantibody in 56128 blood donors, from March to December in 2016. Positive samples were then double tested with blood from blood bag, rechecked positive samples were confirmed by electrochemiluminescence method （ECL） and Western blot （WB）. Results Among the 56128 blood donors, 20 were screened positive, 5 cases were confirmed positive, the positive rate was 0.01%.Five confirmed positive blood donors were all male, and three of them were both positive for ECL and WB. Conclusion The results showed that in Huizhou area ,HTLV-Ⅰ/Ⅱ infection exist in blood donors, for the safety of blood transfusion, it is necessary to screen HTLV in blood donors.