油菜秸秆高温水解液中单糖组分的高效液相色谱测定

Determination of Monosaccharides in High Temperature Hydrolysate of Rape Straw by High Performance Liquid Chromatography

建立了高温水中CO2催化油菜秸秆水解体系中单糖的高效液相色谱(HPLC)分析的方法。其分析采用PrevailTM ES糖柱,蒸发光散射检测器(ELSD),流动相为乙腈/水(体积比80∶20),等度洗脱,流速0.8 m L/min,柱温35℃,漂移管温度95℃,干燥空气作为载气,流速为3.0 L/min。20 min内阿拉伯糖、木糖、果糖、甘露糖、半乳糖和葡萄糖6种单糖得到了较好的分离,各物质线性回归方程的相关系数R2为0.999 1~0.999 7,其平均回收率为95.3%~108.0%,相对标准偏差(RSD)均小于3%。

The method was developed to determine monosaccharides in the high temperature hydrolysate of rape straw catalyzed by CO2 via high performance liquid chromatography（ HPLC）. The monosaccharides were separated on the PrevailTMES carbohydrate column at 35 ℃ with the mixture of acetonitrile/water（ 80∶20,volume ratio） as mobile phase,which was under isocratic elution at the flow rate of 0. 8 m L/min. They were detected by evaporative light-scattering detector（ ELSD） using dry air as carrier gas at flow rate of 3. 0 L/min and drift tube working at 95 ℃. The HPLC analysis method could ensure six monosaccharides（ including arabinose,xylose,fructose,mannose,galactose and glucose） separate well within 20 min. The resultant correlation coefficients（ R2） of monosaccharides were 0. 999 1-0. 999 7. The average recovery values were 95. 3 %-108. 0 %,and the relative standard deviation percentage（ RSD） was less than 3 %.