益气养阴祛瘀中药对干燥综合征NOD小鼠颌下腺TLR-IFN-BAFF信号通路的影响

Effect of Chinese Herbs for Qi Benefiting,Yin Nourishing,Stasis Removing on TLR-IFN-BAFF Signaling Pathway in Submaxillary Gland of NOD Mice with Sjgren′s Syndrome

目的通过观察益气养阴祛瘀中药对NOD小鼠颌下腺组织中Toll样受体-5(TLR5)、α干扰素(IFN-α)、B淋巴细胞刺激因子(BAFF)蛋白表达的影响,以此来探究益气养阴祛瘀中药对干燥综合征(Sj9gren′s Syndrome,SS)的治疗机制。方法将30只8周龄NOD小鼠随机分为模型组、羟氯喹组和中药组,每组各10只。另将10只8周龄ICR小鼠作为正常对照组(正常组)。正常组和模型组正常饲养,羟氯喹组每日给予羟氯喹75mg/kg灌胃治疗;中药组每日给予益气养阴祛瘀中药煎剂(27.4g/kg)灌胃治疗。饲养用药12周后,各组小鼠股动脉取血,断颈处死,摘取颌下腺组织;通过Western blot法检测各组小鼠颌下腺组织中TLR5、IFN-α和BAFF蛋白表达水平。结果模型组NOD小鼠颌下腺组织中的TLR5、IFN-α和BAFF蛋白相对表达量较正常组ICR小鼠均升高(P<0.05);与模型组比较,中药组小鼠颌下腺组织中的TLR5、IFN-α、BAFF蛋白相对表达量降低(P<0.05)。与羟氯喹组比较,中药组NOD小鼠的IFN-α蛋白相对表达量降低(P<0.05)。结论益气养阴祛瘀中药对SS样NOD小鼠的治疗机制可能与调控TLR-IFN-BAFF信号通路有关。

Objective To observe the effect of Chinese herbs for qi benefiting, yin nourishing, stasis removing （CH-QBYNSR） on expressions of Toll-like receptor 5 （TLR5） ,interferon oL （IFN-α） and BAFF （B cell activating factor belonging to the TNF family） in submaxillary gland of NOD mice, thus studying its therapeutic mechanism for treating Sjsgren＇s syndrome （SS）. Methods Totally 30 8-week-old NOD mice were randomly divided into model group, hydroxychloroquine group, and CH group, 10 in each group. Another ten 8-week-old ICR mice were recruited as a normal group. Mice in the normal group and the model group were normally fed. Hydroxychloroquine （75 mg/kg per day） was administered to those in the hydroxychloroquine group by gastro- gavage. CH-QBYNSR was administered to those in the CH group by gastrogavage （27.4 g/kg per day）. After 12 weeks of feeding and medication, mice were sacrificed by neck dissection, and blood collected from femoral artery. The submandibular gland tissue was collected. Protein expression levels of TLR5, IFN-（x, and BAFF in submandibular gland tissue of mice were detected by Western blot. Results Compared with the normal group, the relative expression levels of TLR5, IFN-α, and BAFF in submandibular gland tissue of NOD mice were in- creased （P 〈0.05）. Compared with the model group, the relative expression levels of TLR5, IFN-α, and BAFF in submandibular gland tissue of the CH group were decreased （P 〈0.05）. Compared with the hydroxychloro- quine group, the relative expression level of IFN-α in submandibular gland tissue of NOD mice were decreased （P 〈0. 05）. Conclusion The therapeutic mechanism of CH-QBYNSR for NOD mice was associated with regula- ting TLR-IFN-BAFF signaling pathway.