白细胞介素-17基因启动子区域甲基化与宫颈癌的关系研究

Study on relationship of promoter region methylation of IL-17 gene and cervical cancer

目的检测宫颈癌白细胞介素17(interleukin-17,IL-17)基因启动子区域甲基化的变化,探讨其相关的临床意义。方法选取2015年2月至2017年3月黄石市中医医院妇产科收治的宫颈癌患者手术切除的宫颈癌组织87例为宫颈癌组,选取同期因功血、子宫肌腺病等子宫良性病变切除的正常宫颈组织40例为对照组。IL-17基因启动子甲基化使用亚硫酸盐修饰后聚合酶链式反应检测。结果 87例宫颈癌组织标本中检测到IL-17基因启动子甲基化阳性率为17.2%,显著低于对照组的42.5%(P<0.05)。IL-17基因启动子甲基化阳性率在宫颈癌患者不同年龄和组织类型之间差异无统计学意义(P>0.05)。但IL-17基因启动子甲基化阳性率在FIGO临床分期Ⅲ、Ⅳ期患者为10.9%,显著低于Ⅰ、Ⅱ期患者的24.4%(P<0.05)。IL-17基因启动子甲基化阳性率在有淋巴结转移患者为12.2%,显著低于无淋巴结转移患者的23.7%(P<0.05)。IL-17基因启动子甲基化阳性率在中、低分化患者为12.5%,显著低于高分化患者的25.8%(P<0.05)。结论 IL-17基因启动子在宫颈癌组织中呈现低甲基化状态,与宫颈癌的临床分期、有无淋巴结转移以及肿瘤分化程度密切相关。

Objective To detect promoter region methylation of interleukin - 17 （ IL - 17） gene in cervical cancer and explore its clinical significance. Methods 87 cervical cancer tissue resected in surgery from patients with cervical cancer in Huangshi Chinese Medicine Hospital from February 2015 to March 2017 were selected. 40 normal cervical tissue resected from patients with dysfunctional uterine bleeding, uterine adenomyosis and other benign uterine lesions were selected as control. IL - 17 gene promoter methylation was detected using a sulfite modified polymerase chain reaction assay. Results The positive rate of IL - 17 gene promoter methylation in cervical cancer patients was 17.2% , which was significantly lower than that of 42. 5% in control group（P 〈 0. 05）. There was no significant difference of positive rate of IL - 17 gene promoter methylation between different ages and tissue type in cervical cancer（P 〉 0. 05 ）. The positive rate of IL - 17 methylation in Ⅲ and Ⅳstage cervical cancer group was 10. 9% , which was lower than that of 24. 4% in Ⅰ and Ⅱstage cervical cancer group（P 〈0. 05）. The positive rate of IL- 17 methylation in lymph node metastasis group was 12. 2% , which was lower than that of 23.7% in non - lymph node metastasis group （P 〈 0. 05 ）. The positive rate of IL - 17 methylation in median and low differentiation group was 12. 5%, which was lower than that of 25.8% in well differentiation group group（P 〈 0. 05 ）. Conclusion Lower- methylation of IL- 17 was detected in cervical cancer, which was related to clinical stages, lymph node metastasis and tumor differentiation of cervical cancer.