桔梗提取物的主要成分和抗氧化性的测定

Detection of Main Components and Antioxidant Activity of Platycodon grandiflorum Extracts

为评价桔梗提取物中主要成分的含量和体外抗氧化性,通过回流提取法制备桔梗水提物和醇提物,水提醇沉法制备桔梗多糖;高效液相色谱法检测桔梗皂苷D含量,亚硝酸钠-硝酸铝比色法检测总黄酮含量,硫酸-苯酚法检测多糖含量;采用分光光度法检测不同提取物清除羟基和DPPH自由基的能力。结果表明:安徽和吉林产地桔梗醇提物中总黄酮在生药中含量分别为1.35%和1.30%,均高于陕西桔梗1.08%,安徽和吉林产地桔梗醇提物对DPPH·和·OH的清除率均高于陕西桔梗醇提物;安徽、吉林和陕西桔梗多糖在生药中含量分别为0.45%、0.39%和0.35%。3个产地桔梗多糖对DPPH·和·OH的清除作用安徽最高,吉林次之,陕西最低。桔梗醇提物对DPPH·和·OH的清除作用均高于桔梗水提物和桔梗多糖对DPPH·和·OH的清除作用。试验认为,桔梗醇提物对于DPPH·和·OH均具有较好的清除作用,以期为开发相关的抗氧化性产品提供参考。

The paper aims to evaluate main components and antioxidant activity of Platycodon grandiflorum extracts. Water and ethanol extracts were prepared by reflux extraction, polysaccharide was prepared by waterextracting-alcohol precipitating method. Platycodin D content was detected by HPLC method,spectrophotometry was used to detect the content of total flavonoids and polysaccharide. The antioxidantactivities of the extracts were detected based on the clearance of hydroxyl radical and DPPH · model. Thecontent of total flavonoids in ethanol extracts from Anhui, Jilin and Shaanxi was 1.35%, 1.30% and 1.08%,respectively. The clearance rate for scavenging DPPH· and ·OH of ethanol extracts from Anhui and Jilin washigher than that from Shaanxi. The content of polysaccharide in water extracts from Anhui, Jilin and Shaanxiwas 0.45%, 0.39% and 0.35%, respectively. The clearance rate of polysaccharide for scavenging DPPH· and ·OHfrom Anhui was the highest, that from Jilin was the next, and that from Shaanxi was the lowest. The clearancerate of P. grandiflorum ethanol extracts for scavenging DPPH· and ·OH was higher than that of water extractsand polysaccharide. Ethanol extracts have good in vitro antioxidant properties and provide some reference for the development of related antioxidant products.