不同类型甜瓜亲本及其F1代果实挥发性物质成分的比较

Volatile Organic Compounds Comparision Among Different Genotype of Melon and Their Hybrids

以野生薄皮甜瓜("马泡",杂交父本)、厚皮网纹甜瓜(杂交母本)和二者杂交的F1代果实为试材,利用气相色谱-质谱联用(GC-MS)技术检测其成熟果实中挥发性物质的种类和相对含量的差异。结果表明:网纹厚皮甜瓜、野生甜瓜和F1检测到的挥发性物质成分分别为50、70、79种,3个不同类型甜瓜的果实中共检测出120种挥发性物质,其中包括58种酯类、10种醇类、4种醛类、12种烯类和36种其它物质;3个类型甜瓜的果实中共同含有的挥发性物质有23种。3个类型甜瓜的果实中主要的挥发性物质均为酯类物质,但种类和相对含量存在差异。其中F1代挥发性物质最为丰富,表明挥发性物质的种类具有超亲优势的潜力,该结果为研究甜瓜挥发性物质代谢提供一定的参考依据。

The volatile organic compounds （VOCs） of wild melon （＇Mapao＇, male parent）, netted muskmelon （female parent） and their F1 were analyzed with the method of head space-soild phase microextraction and Gas Chormatography-Mass Spectrometry （GC-MS）. The results indicated that the number of VOCs identified from netted muskmelon, wild melon and F1 fruits were 50, 70 and 79, respectively. And total 120 kinds of VOCs in the melon were identified. The major VOCs in melon consisted of 58 esters, 10 alcohols,4 aldehydes, 19 alkenes and 36 other substances. These three melon types shared 23 same VOCs. The main VOCs in the three types of melon fruit were esters, but the VOCs kinds and relative contents were different. VOC kinds were most abundant in F1 melon fruit, indicating VOCs had the potential of heterosis over better parents. The results provided a basis for studying VOCs metabolism in melon fruit.