摘要
目的:探讨山茱萸提取物对原发性肝癌(hepatocellular carcinoma,HCC)大鼠B7-H6表达的影响。方法:选取60只SD大鼠随机分为模型组、苦参碱组和山茱萸组,以二乙基亚硝胺诱发大鼠模型,分别给予苦参碱组和山茱萸组大鼠相应药物灌胃,模型组大鼠给予0.9%Na Cl溶液灌胃。计数各组大鼠肝脏表面的癌结节数及其抑瘤率;经H&E染色后观察各组大鼠肝癌组织的病理学改变。应用免疫组织化学法和Western blot法检测模型组、苦参碱组和山茱萸组大鼠肝癌组织中B7-H6的表达。结果:苦参碱组和山茱萸组大鼠的肝癌结节数明显少于模型组(P<0.05);山茱萸组的抑瘤率显著高于苦参碱组(P<0.05)。免疫组织化学结果显示,山茱萸组和苦参碱组肝癌组织中B7-H6的阳性表达显著高于模型组(P<0.05),山茱萸组B7-H6的阳性表达显著高于苦参碱组(P<0.05)。Western blot法结果显示,山茱萸组和苦参碱组B7-H6的蛋白表达显著高于模型组(P<0.05),山茱萸组B7-H6的蛋白表达显著高于苦参碱组(P<0.05)。结论:山茱萸提取物可能通过上调肿瘤组织B7-H6的表达,抑制大鼠肝癌组织的生长。
Objective:To investigate the effects of Fructus Corni extract on the B7-H6 expression in primary liver cancer cells of rats.Methods:Sixty SD rats were randomly divided into three groups,namely,model,matrine,and Cornus officinalis.The rat model bearing the primary liver cancer was induced by diethylnitrosamine,except for the rats in the control group.The rats in both the matrine and Cornus officinalis groups were fed with matrine and Cornus officinalis.The rats in model groups were fed with 0.9% sodium chloride solution.The number of hepatocellular carcinoma nodules was calculated,and the tumor growth inhibition rate was also calculated.The pathological changes of hepatic tissues in rats of each group were observed by hematoxylin and eosin staining.The expression levels of B7-H6 in these three groups were determined by immunohistochemistry and Western blot.Results:The number of liver nodules of the matrine and Fructus Corni group rats was lower than that of the model group(P〈0.05).The tumor inhibition rate of the Cornus group was significantly higher than that of the matrine group(P〈0.05).The tumor growth inhibition rate of the Cornus officinalis group was significantly higher than that of the matrine group(P〈0.05).Immunohistochemistry showed that the positive expression of B7-H6 in the Cornus officinalis group and the matrine group was significantly higher than that in the model group(P〈0.05),and the positive expression of B7-H6 in the Cornus officinalis group was significantly higher than that in the matrine group(P〈0.05).Similarly,the protein expression of B7-H6 in the Cornus officinalis and matrine groups was significantly higher than that in the model group(P〈0.05) by Western blot,while the protein expression of B7-H6 in the Cornus officinalis group was significantly higher than that in the matrine group(P〈0.05).Conclusion:Fructus Corni extract may inhibit the growth of hepatocellular carcinoma through upregulating the B7-H6 expression.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2017年第22期1125-1129,共5页
Chinese Journal of Clinical Oncology