摘要
以蛹虫草全基因组为模板查找Simple Sequence Repeats(SSR)位点并设计筛选引物进行聚类分析。从100对SSR引物中选出30对多态性高、重复性好的SSR引物,共获得215条多态性条带。根据遗传相似系数(GS)采用不加权成对群算术平均法(UPGMA)进行遗传相似性聚类:材料间GS值的变化范围为0.527~0.995,以GS值为0.761水平可将46株蛹虫草分为6类;菌株亲缘关系与地域来源有一定相关性,且栽培菌株多为同一品种。
The Cordyceps militaris genome was used as template to identify the SSR loci,to design the SSR primers,and the subsequent repeatable polymorphic bands were utilized for cluster analysis. The results showed that 30 SSR primers generated 215 highly repeatable polymorphic bands. Based on the estimation of genetic similarity coefficient（GS）calculated with unweighted pair group method arithmetic average（UPGMA）clustering method,the variation range of GS values were from 0.527 to 0.995,and 46 C. militaris strains could be divided into 6 groups with a cutoff of the GS value of 0.761. There was a certain correlation between the genetic relationship and the geographical origin of the strains. Interestingly,the strains used for commercial cultivation were genetically similar to each other.
出处
《园艺学报》
CAS
CSCD
北大核心
2017年第11期2195-2202,共8页
Acta Horticulturae Sinica
基金
安徽省2015年高等学校省级质量工程项目(2015ckjh027)
上海市科委平台建设项目(16DZ2281300)
