某型血细胞分离机最适校准系数的研究

One type blood cell separator's optimal calibration coefficient

目的通过比较不同校准系数(YSF系数)下Trima Accel型血细胞分离机采集的单采血小板Plt含量,探寻该机器在实际应用中的最适校准系数。方法选取Trima Accel型血细胞分离机,将调节不同的优化YSF系数后的机器作为3个实验组,保持原YSF系数的机器作为对照组。每台血细胞分离机完成采集110例并记录每份血小板的Plt检测值,重量,通过公式计算得出其实际血小板含量。将4组数据进行统计分析,比较不合格率与高值率,绘制正态分布曲线,选出最合适的血小板YSF系数。结果应用完全随机设计的方差分析得出使用相同YSF系数的不同机器之间的差异无统计学意义(P>0.05);每一实验组与对照组的参数设置对单采血小板Plt含量有显著影响,二者相比有统计学意义(P<0.05)。应用YSF系数为0.98的机器采集出的单采血小板Plt含量为(2.76±0.22)×1011/袋,不合格率为14.55%(16/110),高值率为20.00%(22/110);应用YSF系数为0.99的机器采集出的单采血小板Plt含量为(2.69±0.18)×1011/袋,不合格率为14.55%(16/110),高值率为12.73%(14/110);应用YSF系数为1.00的机器采集出的单采血小板Plt含量为(2.65±0.23)×1011/袋,不合格率为22.73%(25/110),高值率为13.64%(15/110);应用YSF系数为1.01的机器采集出的单采血小板Plt含量为(2.59±0.19)×1011/袋,不合格率为33.64%(37/110),高值率为3.64%(4/110)。再经过正态分布曲线比较后得出的最适YSF系数为0.99。结论 Trima Accel型血细胞分离机在YSF系数为0.99时,离散程度最小,合格率符合国家标准,高值率处于最佳水平,可优化血小板的采集过程,提高血小板单采的综合质量。

Objective To record the solid content of platelet by different calibration coefficients（ YSF coefficient） via blood cell separator collection of Trima Accel type,to compare the difference,and to explore the machine＇s optimal calibration coefficient in practical application at blood stations. Methods The type of Trima Accel blood cell separator was selected,then the machinery was adjusted using different optimized coefficient of YSF,finally they were divided into three experimental groups. The original YSF coefficient of the machine was kept as the control group. Every blood cell separator machinery completed 50 cases,recorded each platelet Plt readings and weight,and calculated the actual content of platelets through the formula. The four groups underwent the statistical analysis,in which fraction defective rate and high probability were estimated. A normal distribution curve was drawn,and the most suitable platelet YSF coefficient was selected. Results The design was completely randomized and the same YSF coefficient between different machines had no differences so it did not have statistical significance（ P〉0. 05）. Each parameter of the experimental group and control group played a significant effect on the solid content of platelet Plt. The comparison between the two was statistically significant（ P〈0. 05）. The machinery which YSF coefficient was 0. 98 collected（ 2. 76± 0. 22） × 10^11/bag Plt. The percent of pass was 14. 55%,high value at a rate of20. 00%. The machinery which YSF coefficient was 0. 99 collected（ 2. 69 ± 0. 18） × 10^11/bag Plt. The percent of pass was14. 55%,the high value at a rate of 12. 73%. The machinery which YSF coefficient was 1. 00 collected（ 2. 65±0. 23） ×10^11/bag Plt. The percent of pass was 22. 73%,high value at a rate of14. 55%. The machinery which YSF coefficient was 1. 01 collected（ 2. 59±0. 19） × 10^11/bag Plt. The percent of pass was33. 64%,high value at a rate of 3. 64%. Through the normal distribution curve,the optimal YSF coefficient was estimated at0. 99. Conclusion When the Trima Accel blood cell separator＇ s YSF coefficient is 0. 99,the discrete degree is the minimum and the qualified rate is in line with national standards,high value rate is also at optimum levels. Platelet collection process can be optimized,which can optimize the acquisition process and improve the comprehensive quality of solid platelet.