苦参素胶囊对BALB/c小鼠人肝癌细胞株SMMC-7721体外种植瘤的影响

Effect of Marine Capsules on BALB/c Human Hepatocellular Carcinoma Cell Line SMMC-7721 in vitro

目的:观察苦参素胶囊对BALB/c小鼠人肝癌细胞株SMMC-7721体外种植瘤的影响。方法:采用体外传代培养人肝癌SMMC-7721细胞株,建立体外种植瘤小鼠肝癌模型。随机分为模型组、氟尿嘧啶(5-Fu)组(25 mg·kg^(-1))、苦参素胶囊高剂量组(90 mg·kg^(-1))、苦参素胶囊低剂量组(45 mg·kg^(-1)),另设空白对照组。每组10只,连续给药14 d后。眼球取血,分离血清,Elisa法测定IL-2水平。摘取瘤株,称量湿质量,计算抑制率;HE染色,观察肿瘤组织的病理改变。结果:与模型组比较,苦参素胶囊高、低剂量组可显著升高小鼠血清IL-2含量(P<0.01或P<0.05);抑制体外种植瘤生长(P<0.001或P<0.05);高剂量组上述作用与5-Fu组相比无明显差异(P>0.05)。HE染色可见小鼠肿瘤细胞核染色变浅,肿瘤细胞数量减少。结论:苦参素胶囊对BALB/c小鼠人肝癌细胞株体外种植瘤有一定的抑制作用。

Objective： To observe the effect of marine capsules on BALB/c human hepatocellular carcinoma cell line SMMC-7721 in vitro. Methods： Human hepatoma SMMC-7721 cell line was cultured in vitro to establish a hepatoma tumor model, and was randomly divided into the model control group, 5-Fu group （25 mg·kg^-1）, marine capsules high dose group （90 mg·kg^-1）, marine capsules low dose group （45 mg·kg^-1） and the blank control group with 10 mice in each and 14-day continuous administration. Blood was taken from the eyeball and serum was isolated. The tumor strains were picked, the wet weight was obtained, the inhibition rate was calculated, and the pathological changes of tumor tissues were observed after HE staining. Results ： Compared with that in the model control group, the serum IL-2 content significantly increased in the marine capsules high and the low dose groups （P 〈0.01, P 〈0. 05）. The in vitro growth of tumor （P 〈 0. 001, P 〈 0.05） was inhibited with the inhibition rate of 41.56% and 27.78% respectively for the high dose group and the low dose group, and the effects of the high dose group had no significant differences with those of 5-Fu group （ P 〉 0.05 ）. The nuclear staining of tumor cells was lighter and the number of tumor cells decreased in the two marine capsules groups. Conclusion： Marine capsules have certain inhibitory effects on BALB/c human hepatocellular carcinoma cell line SMMC-7721 in vitro.