星点设计-效应面法优化镰形棘豆总生物碱的超声提取工艺

Optimization of Ultrasonic Extraction Process of Total Alkaloids from Oxytropis Falcata Bunge by Central Composite Design and Response Surface Method

目的:优选镰形棘豆总生物碱的提取工艺。方法:在单因素考察的基础上,选择溶剂用量、超声时间、乙醇体积分数为自变量,以镰形棘豆中总生物碱含量为因变量,采用星点设计-效应面法优选出镰形棘豆总生物碱的最佳提取工艺。结果:最佳提取工艺条件为加入36倍量72%乙醇(含1%乙酸),在60℃下超声提取2次,每次77 min,总生物碱的含量为2.793 mg·g^(-1),与预测值比较偏差小于2%。结论:星点设计-响应面法优化镰形棘豆总生物碱的提取工艺方法简单,精密度高,模型可靠,可预测性趋势良好。

Objective： To optimize the ultrasonic extraction process of total alkaloids from Oxytropis falcata bunge. Methods： The independent variables were solvents ratio, extracting time and ethanol concentration, and the dependent variable was content of total alkaloids, Based on single factor tests, central composite design and response surface methodology was adopted to optimize the extraction technology, Results： The optimal extraction conditions were as follows： extracted twice with 36-fold amount of 72% ethanol （contai- ning 1% acetic acid） at 60℃, and extracted 77 minutes each time. Under the above conditions, the content of total alkaloids was 2.793 mg·g^-1 with the bias ratio less than 2% when compared with the model predictions. Conclusion： Ultrasonic extraction process of total alkaloids from Oxytropisfalcata Bunge optimized by central composite design and response surface method is simple, highly precise, reliable and predictable.